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Rapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001305. Experiment: UPLC9_RJC_DEV_HeLa_03, file: 20140418_QE7_UPLC9_RJC_DEV_HeLaFrac10_03.mzml. Published as part of J Proteome Res. 2014 Oct 28 . From the Abstract: {{i}} Here, we investigate faster sequencing speed of the latest Q Exactive HF mass spectrometer, which features an ultra-high-field Orbitrap mass analyzer. Proteome coverage is evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human_female

DISEASE(S): Not Available

SUBMITTER: Kelstrup CD, et al.  

PROVIDER: GPM11210030961 | GPMDB |

REPOSITORIES: GPMDB

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Rapid and deep proteomes by faster sequencing on a benchtop quadrupole ultra-high-field Orbitrap mass spectrometer.

Kelstrup Christian D CD   Jersie-Christensen Rosa R RR   Batth Tanveer S TS   Arrey Tabiwang N TN   Kuehn Andreas A   Kellmann Markus M   Olsen Jesper V JV  

Journal of proteome research 20141110 12

Shotgun proteomics is a powerful technology for global analysis of proteins and their post-translational modifications. Here, we investigate the faster sequencing speed of the latest Q Exactive HF mass spectrometer, which features an ultra-high-field Orbitrap mass analyzer. Proteome coverage is evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments (ProteomeXchange data set PXD001305). We find the ultra-high-field Orbitrap mass analyzer  ...[more]