Refined phosphopeptide enrichment by phosphate additive and the analysis of human brain phosphoproteome.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001180. File: tio2_250ug.mzml. Published as part of Proteomics. 2015 Jan;15(2-3):500-7 . From the Abstract: {{i}} Here, we report a pilot investigation of AD phosphoproteome by titanium dioxide enrichment coupled with high resolution LC-MS/MS. During the optimization of the enrichment method, we found that phosphate ion at a low concentration (e.g. 1 mM) worked efficiently as a nonphosphopeptide competitor to reduce background. {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human

DISEASE(S): Not Available

SUBMITTER: Haiyan Tan, et al.  

PROVIDER: GPM11210034774 | GPMDB |

REPOSITORIES: GPMDB