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Refined phosphopeptide enrichment by phosphate additive and the analysis of human brain phosphoproteome.


ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001180. File: tio2_250ug.mzml. Published as part of Proteomics. 2015 Jan;15(2-3):500-7 . From the Abstract: {{i}} Here, we report a pilot investigation of AD phosphoproteome by titanium dioxide enrichment coupled with high resolution LC-MS/MS. During the optimization of the enrichment method, we found that phosphate ion at a low concentration (e.g. 1 mM) worked efficiently as a nonphosphopeptide competitor to reduce background. {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human

DISEASE(S): Not Available

SUBMITTER: Haiyan Tan, et al.  

PROVIDER: GPM11210034774 | GPMDB |

REPOSITORIES: GPMDB

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Publications

Refined phosphopeptide enrichment by phosphate additive and the analysis of human brain phosphoproteome.

Tan Haiyan H   Wu Zhiping Z   Wang Hong H   Bai Bing B   Li Yuxin Y   Wang Xusheng X   Zhai Bo B   Beach Thomas G TG   Peng Junmin J  

Proteomics 20141215 2-3


Alzheimer's disease (AD) is the most common form of dementia, characterized by progressive loss of cognitive function. One of the pathological hallmarks of AD is the formation of neurofibrillary tangles composed of abnormally hyperphosphorylated tau protein, but global deregulation of protein phosphorylation in AD is not well analyzed. Here, we report a pilot investigation of AD phosphoproteome by titanium dioxide enrichment coupled with high resolution LC-MS/MS. During the optimization of the e  ...[more]

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