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Omics score: 1008

A double-barrel LC-MS/MS system to quantify 96 interactomes per day.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001695. File: 20140827_EXQ00_FaHo_SA_SWI3_03.mzml. Published as part of Mol Cell Proteomics. 2015 Apr 17 . From the Abstract: {{i}} ... Here we develop a high-throughput workflow capable of dealing with large numbers of low or medium complexity samples and specifically aim at the analysis of 96-well plates in a single day (15 min per sample) ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Yeast

DISEASE(S): Not Available

SUBMITTER: Hosp F, et al.

PROVIDER: GPM11210038526 | GPMDB |

REPOSITORIES: GPMDB

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A Double-Barrel Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) System to Quantify 96 Interactomes per Day.

Hosp Fabian F Scheltema Richard A RA Eberl H Christian HC Kulak Nils A NA Keilhauer Eva C EC Mayr Korbinian K Mann Matthias M

Molecular & cellular proteomics : MCP 20150417 7

The field of proteomics has evolved hand-in-hand with technological advances in LC-MS/MS systems, now enabling the analysis of very deep proteomes in a reasonable time. However, most applications do not deal with full cell or tissue proteomes but rather with restricted subproteomes relevant for the research context at hand or resulting from extensive fractionation. At the same time, investigation of many conditions or perturbations puts a strain on measurement capacity. Here, we develop a high-t ...[more]

PMID: 25887394

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