Gel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD000311. Enzyme: trypsin, file: F017572.dat-ACA-SDS_T3.mzml. Published as part of Proteomics. 2014 Jul 10 . From the Abstract: {{i}} ... Proteins were extracted from pooled whole mouse brains, enriched membrane fractions were prepared using either two commercially available kits or 6-aminocaproic acid under denaturing or native conditions followed by gel-based proteomic approaches using blue native (BN-) and SDS-PAGE with subsequent in-gel digestion with several proteases, chymotrypsin, trypsin followed by nano-LC-ESI-MS/MS analysis on LTQ Orbitrap Velos Pro ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mus_musculus_viruses, Mouse

DISEASE(S): Not Available

SUBMITTER: Heo S, et al.  

PROVIDER: GPM32310002988 | GPMDB |

REPOSITORIES: GPMDB