DUSP2 dephosphorylates STAT3 at Y705 and S727 residues

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD002743. File: DUSP2.mzml. From ProteomeXchange: {{i}} In the related study, to determine whether DUSP2 definitively served as a phosphatase for STAT3, an in vitro phosphatase assay was used. Using S-tag beads, p-STAT3 was pulled down from IL-6 stimulated HEK293T cells transfected with STAT3-S-Tag. DUSP2 or control protein, which was purified from HEK293T cells transfected with DUSP2-FLAG or empty vector through extraction with anti-FLAG beads and elution with FLAG peptides, were incubated with p-STAT3-S-Tag-beads in phosphatase buffer. Then bound proteins were eluted and subjected to MS analysis ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human_female

DISEASE(S): Not Available

SUBMITTER: Dan Lu, et al.  

PROVIDER: GPM32310025099 | GPMDB |

REPOSITORIES: GPMDB