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Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD000666. File: 20120710_EXQ4_DaHo_SA_labelfree_7D_MN1_3_12_2.mzml. Published as part of Mol Cell Proteomics. 2014 Sep 5 . From the Abstract: {{i}} The fatal neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA) are the most common motoneuron disease and genetic cause of infant death, respectively. Various in vitro model systems have been established to investigate motoneuron disease mechanisms - in particular immortalized cell lines and primary neurons. By quantitative mass spectrometry (MS)-based proteomics we here compare the proteomes of primary motoneurons to motoneuron-like cell lines NSC-34 and N2a as well as to non-neuronal control cells at a depth of 10,000 proteins ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mus_musculus_viruses, Mouse

DISEASE(S): Not Available

SUBMITTER: Hornburg D, et al.

PROVIDER: GPM32320003403 | GPMDB |

REPOSITORIES: GPMDB

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Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

Hornburg Daniel D Drepper Carsten C Butter Falk F Meissner Felix F Sendtner Michael M Mann Matthias M

Molecular & cellular proteomics : MCP 20140905 12

The fatal neurodegenerative disorders amyotrophic lateral sclerosis and spinal muscular atrophy are, respectively, the most common motoneuron disease and genetic cause of infant death. Various in vitro model systems have been established to investigate motoneuron disease mechanisms, in particular immortalized cell lines and primary neurons. Using quantitative mass-spectrometry-based proteomics, we compared the proteomes of primary motoneurons to motoneuron-like cell lines NSC-34 and N2a, as well ...[more]

PMID: 25193168

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Dataset Information

Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics

Publications

Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

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