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Omics score: 1004

Site-specific mapping and quantification of protein S-sulphenylation in cells.

ABSTRACT: Data from CPTAC Portal. Experiment: Protein S-sulfenylation SulfenQ mzML_data A431, file: SulfenQ_A431_EGF_LH_B3.mgf. Published as part of Nat Commun. 2014 Sep 1;5:4776 . From the Abstract: {{i}} Cysteine S-sulphenylation provides redox regulation of protein functions, but the global cellular impact of this transient post-translational modification remains unexplored. We describe a chemoproteomic workflow to map and quantify over 1,000 S-sulphenylation sites on more than 700 proteins in intact cells ...{{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human_female

DISEASE(S): Not Available

SUBMITTER: Yang J, et al.

PROVIDER: GPM32320005182 | GPMDB |

REPOSITORIES: GPMDB

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Site-specific mapping and quantification of protein S-sulphenylation in cells.

Yang Jing J Gupta Vinayak V Carroll Kate S KS Liebler Daniel C DC

Nature communications 20140901

Cysteine S-sulphenylation provides redox regulation of protein functions, but the global cellular impact of this transient post-translational modification remains unexplored. We describe a chemoproteomic workflow to map and quantify over 1,000 S-sulphenylation sites on more than 700 proteins in intact cells. Quantitative analysis of human cells stimulated with hydrogen peroxide or epidermal growth factor measured hundreds of site selective redox changes. Different cysteines in the same proteins ...[more]

PMID: 25175731

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Site-specific mapping and quantification of protein S-sulphenylation in cells

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