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Omics score: 1008

Huntingtin associated protein (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP), specific for the cGMP-dependent protein kinase Iβ isoform.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001434. Published as part of J Biol Chem. 2015 Feb 4. pii . From the Abstract: {{i}} ... Here, we extend on this cAMP-based chemical proteomics approach, employing competitive concentrations of free cyclic nucleotides to isolate each kinase and its secondary interactors. Using this approach we identified Huntingtin associated protein 1 (HAP1) as a putative novel GKAP. Through sequence alignment with known GKAPs and secondary structure prediction analysis we defined a small sequence domain mediating the interaction with PKG Iβ, but not PKG I alpha ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human_female

DISEASE(S): Not Available

SUBMITTER: Corradini E, et al.

PROVIDER: GPM32320007631 | GPMDB |

REPOSITORIES: GPMDB

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Huntingtin-associated protein 1 (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP) specific for the cGMP-dependent protein kinase Iβ isoform.

Corradini Eleonora E Burgers Pepijn P PP Plank Michael M Heck Albert J R AJ Scholten Arjen A

The Journal of biological chemistry 20150204 12

Protein-protein interactions are important in providing compartmentalization and specificity in cellular signal transduction. Many studies have hallmarked the well designed compartmentalization of the cAMP-dependent protein kinase (PKA) through its anchoring proteins. Much less data are available on the compartmentalization of its closest homolog, cGMP-dependent protein kinase (PKG), via its own PKG anchoring proteins (GKAPs). For the enrichment, screening, and discovery of (novel) PKA anchoring ...[more]

PMID: 25653285

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