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Omics score: 1006

MS1-Based Label-Free Proteomics Using a Quadrupole Orbitrap Mass Spectrometer.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001385. File: QEP1_SpikeIn_230914_3_3ng_270914.mzml. Published as part of J Proteome Res. 2015 Mar 24 . From the Abstract: {{i}} Presented is a data set for benchmarking MS1-based label-free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences in a background of an unchanged complex proteome. The data set provides a unique opportunity to evaluate the proteomic platform (instrumentation and software) in its ability to perform MS1-intensity-based label-free quantification. {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human_female, Escherichia_coli_k_12_substr__mg1655

DISEASE(S): Not Available

SUBMITTER: Shalit T, et al.

PROVIDER: GPM32320014338 | GPMDB |

REPOSITORIES: GPMDB

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MS1-based label-free proteomics using a quadrupole orbitrap mass spectrometer.

Shalit Tali T Elinger Dalia D Savidor Alon A Gabashvili Alexandra A Levin Yishai Y

Journal of proteome research 20150324 4

Presented is a data set for benchmarking MS1-based label-free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences in a background of an unchanged complex proteome. The data set provides a unique opportunity to evaluate the proteomic platform (instrumentation and software) in its ability to perform MS1-intensity-based label-free quantification. We sh ...[more]

PMID: 25780947

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