Feasibility of Protein Turnover Studies in Prototroph Saccharomyces cerevisiae Strains.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001823. File: PRO_R2_12h.mzml. Published as part of Anal Chem. 2015 Mar 26 . From the Abstract: {{i}} ... Quantitative proteomics studies of yeast that use metabolic labeling with amino acids rely on auxotrophic mutations of one or more genes on the amino acid biosynthesis pathways. These mutations affect yeast metabolism and preclude the study of some biological processes. Overcoming this limitation, it has recently been described that proteins in a yeast prototrophic strain can also be metabolically labeled with heavy amino acids. However, the temporal profiles of label incorporation under the different phases of the prototroph"s growth have not been examined. Labeling trajectories are important in the study of protein turnover and dynamics, in which label incorporation into proteins is monitored across many time points. Here we monitored protein labeling trajectories for 48 h after a pulse with heavy lysine in a yeast prototrophic strain and compared them with those of a lysine auxotrophic yeast ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Yeast

DISEASE(S): Not Available

SUBMITTER: Martin-Perez M, et al.  

PROVIDER: GPM32320014367 | GPMDB |

REPOSITORIES: GPMDB