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Omics score: 1006

Quantitative proteomics analysis of the Arg/N-end rule pathway of targeted degradation in Arabidopsis roots.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001719. Experiment: TAILS_freeNt, file: TAILS_B_freeNt.msf.mgf. Published as part of Proteomics. 2015 Feb 27 . From the Abstract: {{i}} According to the Arg/N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Proteins can also become PRT6 substrates following post-translational arginylation by arginyltransferases ATE1 and 2. Here, we undertook a quantitative proteomics study of Arg/N-end rule mutants, ate1/2 and prt6, to investigate the impact of this pathway on the root proteome. Tandem mass tag (TMT) labelling identified a small number of proteins with increased abundance in the mutants, some of which represent downstream targets of transcription factors known to be N-end rule substrates ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Ath1

DISEASE(S): Not Available

SUBMITTER: Hongtao Zhang, et al.

PROVIDER: GPM32320014396 | GPMDB |

REPOSITORIES: GPMDB

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Quantitative proteomics analysis of the Arg/N-end rule pathway of targeted degradation in Arabidopsis roots.

Zhang Hongtao H Deery Michael J MJ Gannon Lucy L Powers Stephen J SJ Lilley Kathryn S KS Theodoulou Frederica L FL

Proteomics 20150417 14

According to the Arg/N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Proteins can also become PRT6 substrates following post-translational arginylation by arginyltransferases ATE1 and 2. Here, we undertook a quantitative proteomics study of Arg/N-end rule mutants, ate1/2 and prt6, to investigate the impact of this pathway on the root proteome. Tandem mass tag labelling identified a small number of proteins ...[more]

PMID: 25728785

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Quantitative proteomics analysis of the Arg/N-end rule pathway of targeted degradation in Arabidopsis roots

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