Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD002534. File: GR_130129_20_OT3_NT_L3MBTL2_FAB_31_G11_2_HEK293.mzXML. Published as part of Nat Methods. 2015 Jun 29 . From the Abstract: {{i}} ... We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality. We quantified the abundance of all the proteins in immunoprecipitates of 1, 124 new recombinant antibodies for 152 chromatin-related human proteins by comparing normalized spectral abundance factors from the target antigen with those of all other proteins ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Homo_sapiens_viruses, Human_female

DISEASE(S): Not Available

SUBMITTER: Marcon E, et al.  

PROVIDER: GPM32320016573 | GPMDB |

REPOSITORIES: GPMDB