Dataset Information

Grafting watermelon cold stress proteome

ABSTRACT: Rootstock grafting can improve the cold tolerance of watermelon. However, the molecular mechanism underling this process remains unknown. Here, an isobaric tag for relative and absolute quantification based proteomic technique was employed to identify the differentially expressed proteins (DEPs) between pumpkin rootstock-grafted (RG) and self-grafted (SG) watermelon seedlings under cold stress.

ORGANISM(S): Citrullus Lanatus Subsp. Vulgaris

SUBMITTER: Zhilong Bie

PROVIDER: PXD008477 | iProX | Mon Dec 18 00:00:00 GMT 2017

REPOSITORIES: iProX

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Publications

iTRAQ-based quantitative proteomics analysis of cold stress-induced mechanisms in grafted watermelon seedlings.

Shi Xianfeng X Wang Xibo X Cheng Fei F Cao Haishun H Liang Huang H Lu Junyang J Kong Qiusheng Q Bie Zhilong Z

Journal of proteomics 20180927

Rootstock grafting can improve the cold tolerance of watermelon. However, the molecular mechanisms underlying this process remain unknown. Herein, we used an isobaric tag for relative and absolute quantification (iTRAQ)-based quantitative proteomics approach for the comparative analysis of protein abundances in self-grafted (SG) and pumpkin rootstock-grafted (RG) watermelon seedlings in response to cold stress. A total of 4796 distinct proteins were identified, and 752 proteins were significantl ...[more]

PMID: 30267873

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Project description:Background: Rice grain production is susceptible to a changing environment that imposes both biotic and abiotic stress conditions. Cold episodes are becoming more frequent in the last years and directly affect rice yield in areas with a temperate climate. Rice is particularly susceptible to cold stress during the reproductive phase, especially in anthers during post-meiotic stages which, in turn, affect pollen production. However, a number of rice cultivars with a certain degree of tolerance to cold have been described, which may represent a good breeding resource for improvement of susceptible commercial varieties. Plants experiencing cold stress activate a molecular response in order to reprogram many metabolic pathways to face these hostile conditions. Results: Here we performed RNA-seq analysis using cold-stressed post-meiotic anther samples from a cold-tolerant, Erythroceros Hokkaido (ERY), and a cold-susceptible commercial cultivar Sant´Andrea (S.AND). Both cultivars displayed an early common molecular response to cold, although the changes in expression levels are much more drastic in the tolerant one. Comparing our datasets, obtained after one-night cold stress, with other similar genome-wide studies showed very few common deregulated genes, leading to the conclusion that molecular responses in cold-stressed anthers strongly depend on conditions and the duration of the cold treatments. Cold-tolerant ERY exhibits specific molecular responses related to ethylene metabolism, which appears to be activated after cold stress. On the other hand, S.AND cold-treated plants showed a general downregulation of photosystem I and II genes, supporting a role of photosynthesis and chloroplasts in cold responses in anthers, which has remained elusive. Conclusions: Our study revealed that a number of ethylene-related transcription factors, as putative master regulators of cold responses, were upregulated in ERY providing promising candidates to confer tolerance to susceptible cultivars. Our results also suggest that the photosynthesis machinery might be a good target to improve cold tolerance in anthers. In summary, our study provides valuable candidates for further analysis and molecular breeding for cold-tolerant rice cultivars.

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