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Quantitative proteomic analysis of phagocytes in oyster Crassostrea gigas

ABSTRACT: Invertebrates lack the adaptive immune system homologous to that of vertebrates, thus depend exceptionally on the innate immune system for the pathogen recognition and clearance. Phagocytes play a pivotal role in the innate immunity of invertebrates, but the molecular basis underlying their phagocytic killing is still far from well understood. In the present study, phagocytes from the Pacific oyster Crassostrea gigas were efficiently sorted based on the phagocytosis of FITC-labeled latex beads by fluorescence-activated cell sorting. The differentially expressed proteins were revealed by quantitative proteome and validated by qRT-PCR and flow cytometry.

ORGANISM(S): Crassostrea Gigas

SUBMITTER: Linsheng Song

PROVIDER: PXD009987 | iProX | Tue May 29 00:00:00 BST 2018

REPOSITORIES: iProX

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Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.

Jiang Shuai S Qiu Limei L Wang Lingling L Jia Zhihao Z Lv Zhao Z Wang Mengqiang M Liu Conghui C Xu Jiachao J Song Linsheng L

Frontiers in immunology 20180611

As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled lat ...[more]

PMID: 29942306

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