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Immobilization of Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide for the isolation of intact phosphoproteins

ABSTRACT: Phosphorylation of protein plays a key role in the regulation of many biological processes. Herein, a simple approach is developed by immobilization of Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO) as adsorbent for selective isolation of intact phosphoproteins from real samples, such as MCF-7 cell lysate.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yongliang Yu

PROVIDER: PXD010524 | iProX | Fri Jul 20 00:00:00 BST 2018

REPOSITORIES: iProX

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Immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide for selective extraction of phosphoproteins.

Wang Meng-Meng MM Chen Shuai S Zhang Dan-Dan DD Yu Yong-Liang YL Wang Jian-Hua JH

Mikrochimica acta 20181119 12

A sorbent for selective extraction of phosphoproteins was obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit an adsorption capacity of 981 mg g<sup>-1</sup> for β-casein due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups. The results of LC-MS and SDS-PAGE an ...[more]

PMID: 30456423

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