Dataset Information

Conjoint analysis of transcriptional and protein changes in heterosis of hybrid yellow catfish Huangyou-1 uncovers key roles for miRNAs

ABSTRACT: Heterosis is a complex biological phenomenon in which hybridization exhibit superior phenotypic characteristics. The underlying molecular basis for heterosis, particularly for fishes, remains elusive. In this study, we used next-generation sequencing technologies and tandem mass tags to characterise mRNA-seq, miRNA-seq and proteomic of Pelteobagrus fulvidraco, P. vachelli and hybrid yellow catfish Huangyou-1 (P. fulvidraco ♀×P. vachelli ♂) livers and in doing so, offer deeper insight into the transcriptional and protein changes in heterosis uncovers key roles for miRNAs.

ORGANISM(S): Tachysurus Fulvidraco Tachysurus Vachellii Tachysurus Fulvidraco X Tachysurus Vachelli

SUBMITTER: Shaowu Yin

PROVIDER: PXD010728 | iProX | Wed May 15 00:00:00 BST 2019

REPOSITORIES: iProX

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Publications

Integrated Analysis of Transcriptomic, miRNA and Proteomic Changes of a Novel Hybrid Yellow Catfish Uncovers Key Roles for miRNAs in Heterosis.

Zhang Guosong G Li Jie J Zhang Jiajia J Liang Xia X Zhang Xinyu X Wang Tao T Yin Shaowu S

Molecular & cellular proteomics : MCP 20190515 7

Heterosis is a complex biological phenomenon in which hybridization produces offspring that exhibit superior phenotypic characteristics compared with the parents. Heterosis is widely utilized in agriculture, for example in fish farming; however, its underlying molecular basis remains elusive. To gain a comprehensive and unbiased molecular understanding of fish heterosis, we analyzed the mRNA, miRNA, and proteomes of the livers of three catfish species, <i>Pelteobagrus fulvidraco, P. vachelli</i> ...[more]

PMID: 31092672

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Project description:Background: Yellow catfish (Pelteobagrus fulvidraco) is one of the important aquaculture species in China. In recent years, due to the high breeding density, the increasing frequency of feeding, and the excessive addition of feed fat, the excessive deposition of body fat in cultured yellow catfish has become more frequent. MicroRNAs (miRNAs) are an important gene expression regulatory signal molecule that regulates liver fat synthesis and transport and play an important role in fat deposition. However, there is a little research on the mechanism of fatty liver caused by excessive deposition of liver fat. Results: After 60 days of high-fat stress, the growth and feed conversion rate of hybrid yellow catfish (Pelteobagrus fuIvidraco♀×P. vachelli♂) were significantly inhibited, and hepatosomatic index, viscerosomatic index, hepatic triglyceride and cholesterol, and red lipid droplets in liver tissues were increased. Through high-throughput sequencing, we constructed miRNA libraries of high-fat stress at 60d, identified 346 conserved miRNAs and 410 novel miRNAs, among which 13 differentially expressed miRNAs were screened between high-fat diet group and normal-fat diet group. Also, we constructed mRNA transcriptome libraries after high-fat stress. Potential target genes for differentially expressed miRNAs were identified by bioinformatics analysis. Seven miRNA-mRNA pairs were screened. The expression of differential miRNA and mRNA and potential binding sites were analyzed by qRT-PCR and dual luciferase assay. Hybrid yellow catfish could promote the oxidative degradation of liver glucose, reduce fatty acid peroxidation, regulate antioxidant enzyme activity and response of immune and inflammatory to relieve fat deposition and liver stress. Conclusions: The disorders of fat metabolism in liver not only result in feed wastage, increase metabolic burden of yellow catfish, but also cause immune function damage, resulting in a variety of nutritional diseases. The development of this study is to understand the molecular mechanism of hepatic fat deposition in yellow catfish. It has important biological significance for improving protection of liver against stress and healthy culture.

Dataset Information

Conjoint analysis of transcriptional and protein changes in heterosis of hybrid yellow catfish Huangyou-1 uncovers key roles for miRNAs

Publications

Integrated Analysis of Transcriptomic, miRNA and Proteomic Changes of a Novel Hybrid Yellow Catfish Uncovers Key Roles for miRNAs in Heterosis.

Similar Datasets

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