Dataset Information

ITRAQ-based quantitative analysis of age-specific variation in salivary proteome of caries-susceptible individuals

ABSTRACT: Human saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with aging of patients with dental caries. Herein, we applied iTRAQ-based quantitative approach to explore the salivary proteome profile of different aged subjects, with and without caries, aiming to identify age-related markers for dental caries. Unstimulated whole saliva samples were collected from 40 caries-free and caries-susceptible young adults and elderly individuals, and then analyzed by combining iTRAQ isobaric labeling with MRM. Among 658 salivary proteins identified by tandem mass spectrometry, 435 proteins exhibited altered expressions in different age groups with and without caries. Of these, 96 were found to be age-specific among caries-susceptible adults and elders, mainly associated with salivary secretion pathway, while 110 age-specific proteins were identified among the healthy individuals. It was found that the age factor caused significant variations and played an important role in both healthy and cariogenic salivary proteome. Subsequently, a total of 136 target proteins with complex protein-protein interactions, including 14 age-specific proteins associated with caries, were further successfully validated using MRM analysis. Moreover, non-age-specific proteins (histatin-1 and BPI fold-containing family B member 1) were verified to be important candidate biomarkers for common dental caries. Our proteomic analysis established by discovery-through-verification pipeline demonstrated distinct variations caused by age factor in both healthy and cariogenic salivary proteome, highlighting the significance of age in the great potential of saliva for caries diagnosis and biomarker discovery.

ORGANISM(S): Homo Sapiens

SUBMITTER: Linglin Zhang

PROVIDER: PXD011067 | iProX | Wed Sep 12 00:00:00 GMT+01:00 2018

REPOSITORIES: iProX

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iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals.

Wang Kun K Wang Xiuqing X Zheng Sainan S Niu Yumei Y Zheng Wenyue W Qin Xi X Li Zhongcheng Z Luo Junyuan J Jiang Wentao W Zhou Xuedong X Li Wei W Zhang Linglin L

Journal of translational medicine 20181025 1

<h4>Background</h4>Human saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with aging of patients with dental caries. Here, we applied isobaric tags for relative and absolute quantitation (iTRAQ) in combination with multiple reaction monitoring mass spectrometry (MRM-MS) to characterize the salivary proteome profiles of subjects of different ages, p ...[more]

PMID: 30359274

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ITRAQ-based quantitative analysis of age-specific variation in salivary proteome of caries-susceptible individuals

Publications

iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals.

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