Improving Mass Spectrometry Analysis of Protein Structures with Arginine-Selective Chemical Cross-linkers

ABSTRACT: we develop a series of arginine selective bifunctional aromatic glyoxal cross-linkers (ArGOs) and demonstrate their ability to cross-link model proteins and a protein complex. We also introduce the first arginine-lysine heterobifunctional cross-linker (KArGO), which provides significantly greater surface coverage than the corresponding homobifunctional reagents (e.g. ArGOs and BS3), due to access to the combined abundances of lysine and arginine residues.

ORGANISM(S): Escherichia Coli

SUBMITTER: Meng-Qiu Dong  

PROVIDER: PXD012341 | iProX | Mon Jan 14 00:00:00 GMT 2019

REPOSITORIES: iProX