Dataset Information

Thermostable neurotoxins in processed scorpion medicinal material underlie its pharmaceutical basis in traditional Chinese medicine

ABSTRACT: Our work describes the first "map" of functionally active scorpion toxins in processed scorpion medicinal material and provides valuable information for unveiling the pharmaceutical basis for the “combat poison with poison to cure diseases” strategy in traditional Chinese medicine.

ORGANISM(S): Mesobuthus Martensii

SUBMITTER: Xiaolu Zhao

PROVIDER: PXD012392 | iProX | Tue May 07 00:00:00 BST 2019

REPOSITORIES: iProX

ACCESS DATA

Publications

Thermostable potassium channel-inhibiting neurotoxins in processed scorpion medicinal material revealed by proteomic analysis: Implications of its pharmaceutical basis in traditional Chinese medicine.

Yang Fan F Wang Danya D Tong Yongjia Y Qin Chenghu C Yang Liuting L Yu Fei F Huang Xin X Liu Shuang S Cao Zhijian Z Guo Lin L Li Wenxin W Wu Yingliang Y Zhao Xiaolu X

Journal of proteomics 20190704

The neurotoxins of venomous scorpion act on ion channels. Whether these neurotoxins are retained in processed Buthus martensii Karsch scorpions used in traditional Chinese medicine materials is unknown. Comprehensive mass spectrometry-based proteomic characterization of functionally active toxins in the processed medicinal scorpion material revealed 22 full-length and 44 truncated thermostable potassium channel-modulatory toxins that preserved six conserved cysteine residues capable of forming t ...[more]

PMID: 31279926

Similar Datasets

Project description:Chinese medicine is a complex system guided by traditional Chinese medicine (TCM) theories, which has proven to be especially effective in treating chronic and complex diseases. However, the underlying modes of action (MOA) are not always systematically investigated. Herein, a systematic study was designed to elucidate the multi-compound, multi-target and multi-pathway MOA of a Chinese medicine ,QSYQ, on myocardial infarction. Male Sprague Dawley rat model of myocardial infarction were administered QSYQ intragastrically for 7 days while the control group was not treated. The differentially expressed genes (DEGs) were identified from myocardial infarction rat model treated with QSYQ, followed by constructing a cardiovascular disease (CVD)-related multilevel compound-target-pathway network connecting main compounds to those DEGs supported by literature evidences and the pathways that are functionally enriched. Three conditions were compared with three replicates each: (1) sham, i.e. without left anterior descending coronary artery (LAD) ligation; (2) model, with LAD ligation; (3) QSYQ, with LAD ligation and treated with QSYQ intragastrically for 7 days, the dosage was 105.6 mg/kg once a day. Rats were sacrificed after 7 days of i.g. administration under 10% chloral hydrate anesthesia (300mg/kg). Three tissue samples on the border between infarct and non-infarct area were dissected from left ventricles of each group. The tissue samples were stored at -80M-bM-^DM-^C refrigerator. Total RNA was extracted using TRIZol Reagent (Invitrogen) and purified using RNeasy Mini kit (QIAGEN), following manufacturersM-bM-^@M-^Y protocols. RNA quality was evaluated using an Agilent 2100 Bioanalyzer and electrophoresis in 2% (w/v) agarose gels. Only RNA with RNA integrity numbers (RINs) greater than 7.0 and 28SrRNA/18S rRNA ratio greater than 0.7 was used for microarray analyses. Whole genome microarray analysis was performed using Affymetrix rat Genome 230 2.0 chips.

Dataset Information

Thermostable neurotoxins in processed scorpion medicinal material underlie its pharmaceutical basis in traditional Chinese medicine

Publications

Thermostable potassium channel-inhibiting neurotoxins in processed scorpion medicinal material revealed by proteomic analysis: Implications of its pharmaceutical basis in traditional Chinese medicine.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure