Dataset Information

Characterization and discrimination of Tibetan and Duroc × (Landrace × Yorkshire) pork using label-free quantitative proteomics analysis

ABSTRACT: The main aim of this study was to unravel key proteins for the differentiation of Tibetan (n=15) and Duroc × (Landrace × Yorkshire) (n=15) pork. A platform consisting of LC-MS/MS analysis and label-free quantitative proteomics was utilized. Changes in the proteome profile were observed for different pork cuts. A total of 91 and 116 differentially expressed proteins (fold change >2 or <0.5, p-value<0.05) were identified in the five cuts (shoulder, rump, loin, shank and belly) of Tibetan (TP) and Duroc × (Landrace × Yorkshire) (DLY) pork, respectively. Meanwhile, a comparative proteomic analysis was performed between the TP and DLY pork. We identified 102 altered expressed proteins, of which 52.9% (n=54) and 47.1% (n=48) were up- and down-regulated, respectively, in DLY pork compared to TP. Functional analysis of these proteins revealed that the most significantly enriched gene ontology term for biological process was purine-containing compound metabolic process (p=0.003), while that for molecular function was threonine-type peptidase activity (p=0.002), and that for cellular component was mitochondrial inner membrane (p=0.001). The most significantly enriched KEGG pathway was involved in histidine metabolism (p=0.01), followed by oxidative phosphorylation (p=0.02). Using chemometrics approach, we identify 68 significant proteins for the discrimination of TP and DLY pork. The most significantly upregulated proteins in TP and DLY pork were nicotinamide nucleotide transhydrogenase and heat shock protein 90-beta, respectively. This study demonstrates the feasibility of using differential proteomic analysis to discriminate between TP and DLY pork and the current data set can be expanded to a larger sample size for possible discriminant validation.

ORGANISM(S): Sus Scrofa Domesticus

SUBMITTER: Chun-Hui Zhang

PROVIDER: PXD012495 | iProX | Thu Jan 24 00:00:00 GMT 2019

REPOSITORIES: iProX

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Publications

Characterization and discrimination of Tibetan and Duroc × (Landrace × Yorkshire) pork using label-free quantitative proteomics analysis.

Mi Si S Li Xia X Zhang Chun-Hui CH Liu Ji-Qian JQ Huang De-Qiong DQ

Food research international (Ottawa, Ont.) 20190211

The primary aim of this study was to unravel key proteins for the differentiation of Tibetan (n = 15) and Duroc × (Landrace × Yorkshire) (n = 15) pork. A platform consisting of LC-MS/MS analysis and label-free quantitative proteomics was utilized. Changes in the proteome profiles were observed for different pork cuts. A total of 91 and 116 differentially expressed proteins (fold change >2 or < 0.5, p-value<.05) were identified in the five cuts (e.g., shoulder, rump, loin, shank and belly) of Tib ...[more]

PMID: 30884673

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Dataset Information

Characterization and discrimination of Tibetan and Duroc × (Landrace × Yorkshire) pork using label-free quantitative proteomics analysis

Publications

Characterization and discrimination of Tibetan and Duroc × (Landrace × Yorkshire) pork using label-free quantitative proteomics analysis.

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