Ontology highlight
ABSTRACT:
ORGANISM(S): Gossypium Hirsutum
SUBMITTER: Xuchu Wang
PROVIDER: PXD012517 | iProX | Mon Jan 28 00:00:00 GMT 2019
REPOSITORIES: iProX
Jin Xiang X Zhu Liping L Tao Chengcheng C Xie Quanliang Q Xu Xinyang X Chang Lili L Tan Yanhua Y Ding Guohua G Li Hongbin H Wang Xuchu X
BMC genomics 20190411 1
<h4>Background</h4>Two-dimensional electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) are widely used in plant proteomics research. However, these two techniques cannot be simultaneously satisfied by traditional protein extraction methods when investigate cotton leaf proteome.<h4>Results</h4>Here, we evaluated the efficiency of three different protein extraction methods for 2-DE and LC-MS/MS analyses of total proteins obtained from cotton leaves. The protein yie ...[more]