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The essential role of stress granules in cGAS sensing of DNA

ABSTRACT: cGAS-associated cellular proteins through performing co-immunoprecipitation (co-IP) followed by mass spectrometry and proteomic analysis

ORGANISM(S): Homo Sapiens

SUBMITTER: Fei Guo

PROVIDER: PXD012847 | iProX | Sun Feb 24 00:00:00 GMT 2019

REPOSITORIES: iProX

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PKR-dependent cytosolic cGAS foci are necessary for intracellular DNA sensing.

Hu Siqi S Sun Hong H Yin Lijuan L Li Jian J Mei Shan S Xu Fengwen F Wu Chao C Liu Xiaoman X Zhao Fei F Zhang Di D Huang Yu Y Ren Lili L Cen Shan S Wang Jianwei J Liang Chen C Guo Fei F

Science signaling 20191126 609

Cyclic GMP-AMP synthase (cGAS) is a major sensor of cytosolic DNA from invading pathogens and damaged cellular organelles. Activation of cGAS promotes liquid-like phase separation and formation of membraneless cytoplasmic structures. Here, we found that cGAS bound G3BP1, a double-stranded nucleic acid helicase involved in the formation of stress granules. Loss of G3BP1 blocked subcellular cGAS condensation and suppressed the interferon response to intracellular DNA and DNA virus particles in cel ...[more]

PMID: 31772125

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Bloom syndrome protein restrains innate immune sensing of DNA damage by cGAS.

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