Dataset Information

Drosophila proteome

ABSTRACT: Our data describes the first atlas of long-lived proteins in somatic and reproductive tissues of Drosophila during adult lifespan, and reveals a preferential ubiquitylation in the aging proteome.

ORGANISM(S): Drosophila Melanogaster

SUBMITTER: Yaoyang Zhang

PROVIDER: PXD013101 | iProX | Fri Mar 15 00:00:00 GMT 2019

REPOSITORIES: iProX

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Publications

Ubiquitylome study identifies increased histone 2A ubiquitylation as an evolutionarily conserved aging biomarker.

Yang Lu L Ma Zaijun Z Wang Han H Niu Kongyan K Cao Ye Y Sun Le L Geng Yang Y Yang Bo B Gao Feng F Chen Zuolong Z Wu Zhen Z Li Qingqing Q Shen Yong Y Zhang Xumin X Jiang Hong H Chen Yelin Y Liu Rui R Liu Nan N Zhang Yaoyang Y

Nature communications 20190521 1

The long-lived proteome constitutes a pool of exceptionally stable proteins with limited turnover. Previous studies on ubiquitin-mediated protein degradation primarily focused on relatively short-lived proteins; how ubiquitylation modifies the long-lived proteome and its regulatory effect on adult lifespan is unclear. Here we profile the age-dependent dynamics of long-lived proteomes in Drosophila by mass spectrometry using stable isotope switching coupled with antibody-enriched ubiquitylome ana ...[more]

PMID: 31113955

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Project description:In many vertebrate species, age at maturity is proportional to organismal lifespan. A number of theories have attempted to provide a conceptual framework for this fascinating apparent co-evolution. The ‘disposable soma’ theory predicts that costly resources that are invested in germline preservation come at the expense of repairing age-related somatic damage. On the other hand, classical experiments in C. elegans have reported that germline ablation promotes longevity via endocrine signals mediated by the somatic gonad. However, how these seemingly opposing mechanisms may affect vertebrate aging is largely unknown. Here, we use the naturally short-lived turquoise killifish (N. furzeri) to demonstrate that germline ablation can enhance damage repair and promote vertebrate longevity. This was achieved by producing a detailed single-cell atlas of the killifish ovary and testis, and generating a panel of genetic perturbations to manipulate the killifish reproductive state. Specifically, we either block costly stages of germline differentiation by mutating gonadotropins and their receptors, or alternatively, ablate the germline altogether by mutating the dnd1 gene (dead end). Interestingly, only germline ablation enhances somatic repair, particularly in females, by improving tail regeneration following genotoxic stress. To investigate the observed sexual dimorphism, we directly compare several somatic cell types from WT and dnd1 fish. We identify a surprising male-specific upregulation of pro-longevity pathways, including protein homeostasis and stress resistance. Accordingly, only germline-depleted males experience a significant extension in maximal lifespan. Together, dissecting distinct paradigms of germline manipulations, reveals a functional link between the reproductive state, damage repair, and longevity. The sex-specific effect further supports the concept that phenotypes are independent of resource allocation, and instead, could be the result of a possible endocrine effect. Our findings may therefore have exciting implications for uncoupling the reproductive state from vertebrate aging.

Dataset Information

Drosophila proteome

Publications

Ubiquitylome study identifies increased histone 2A ubiquitylation as an evolutionarily conserved aging biomarker.

Similar Datasets

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