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Comparative proteomics analysis of serum protein factors for predicting the prognosis of HBV-related acute-on-chronic liver failure (ACLF)

ABSTRACT: Acute-on-chronic liver failure (ACLF) is an acute deterioration of liver function in patients with hepatitis B virus-related cirrhosis. Numerous risk factors were determined to predict the mortality of HBV-related ACLF. However, little is known about the whole serum proteome profiles in ACLF patients. Here, we perform a comparative proteomics analysis to describe the difference of the proteome profiles between the serum samples from survival (S) and death (D) ACLF patients on the day of hospitalization (H) and after four weeks (F) of treatment in hospital. A total of 518 proteins were identified, of which 295 were considered as high-quality proteins (95% peptides ≥ 2). Together, 112 proteins showed significantly different abundant (DAPs). The GO enrichment analysis showed that the DAPs between S and D group at the day of hospitalization were mostly located in lipoprotein particles and synaptic vesicle. Notably, six proteins showed continuously different abundant between S and D groups, both at H and F time point. We further validated three protein factors for predicting the prognosis of ACLF by ELISA in expanded samples, resulting in a 92.22% AUC for combination of ITIH3 and APO-C1 proteins. These proteins may serve as novel factors for predicting the prognosis of HBV-related ACLF. This study provided an overview of proteome profiles between survived and dead ACLF patients and reveals novel serum factors for predicting the prognosis of this extreme condition.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaozhong Wang

PROVIDER: PXD013582 | iProX | Fri Apr 19 00:00:00 BST 2019

REPOSITORIES: iProX

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