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Identification of MACC1-AS1 binding proteins

ABSTRACT: In vitro RNA synthesis and pull-down assay and MS spectrometry

ORGANISM(S): Homo Sapiens

SUBMITTER: Wei Gu

PROVIDER: PXD014601 | iProX | Fri Jul 12 00:00:00 BST 2019

REPOSITORIES: iProX

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LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1.

Zhang Xiaona X Zhou Yanchun Y Chen Shaoying S Li Wei W Chen Weibing W Gu Wei W

Oncogenesis 20191210 12

Long noncoding RNA (lncRNA) represents a class of endogenous RNAs that regulate gene expression in eukaryotes. To date, the function and underlying mechanism of the majority of mammalian lncRNAs remain unknown. Here, we report that MACC1-AS1, a cognate antisense lncRNA of the sixth intron of the MACC1 gene, functions as a cell growth modulator and enhances breast tumor progress. RNA pulldown and luciferase assays showed that MACC1-AS1 contained binding sites for multiple miRNAs, including well-k ...[more]

PMID: 31822653

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