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Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease

ABSTRACT: Late blight disease (LBD) caused by the pathogen Phytophthora infestans (PI) is the most devastating disease limiting potato production globally. To better understand potato molecular response to LBD, the proteomics and morphological changes of three potato cultivars: Favorita (FA), Mira (MA), and E-malingshu N0.14 (E14) was investigated 5 days after PI inoculation, using iTRAQ-based method. Proteomic analysis of the cultivars resulted in the identification and quantification of a total of 3306 and 2044 proteins. Cluster analysis revealed MA and E14 clustered together separately from FA. Protein expression pattern and related functions revealed the cultivars shared a typical hypersensitive response to PI, including induction of elicitors, oxidative burst, and suppression of photosynthesis. Meanwhile, MA and E14 deployed additional specific responses different from FA, involving high induction of protease inhibitors, serine/threonine kinases, terpenoid, and polyketides, hormone signaling, and transport, which contributed to MA tolerance of LBD. Furthermore, the induction of pathogenesis-related proteins, LRR receptor-like kinases, mitogen-activated protein kinase, WRKY transcription factors, jasmonic acid, and phenolic compounds mediate E14 resistance against LBD. These proteins were confirmed at the transcription level by qPCR and at the translation level by western-blot. Our results provide further insights into the different potato proteomic defense machinery against LBD.

ORGANISM(S): Solanum Tuberosum

SUBMITTER: Chunfang Xiao

PROVIDER: PXD014647 | iProX | Thu Jul 18 00:00:00 BST 2019

REPOSITORIES: iProX

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Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease.

Xiao Chunfang C Huang Mengling M Gao Jianhua J Wang Zhen Z Zhang Denghong D Zhang Yuanxue Y Yan Lei L Yu Xiao X Li Bo B Shen Yanfen Y

BMC genomics 20201209 1

<h4>Background</h4>Late blight disease (LBD) caused by the pathogen Phytophthora infestans (PI), is the most devastating disease limiting potato (Solanum tuberosum) production globally. Currently, this disease pathogen is re-emerging and appearing in new areas at a very high intensity. A better understanding of the natural defense mechanisms against PI in different potato cultivars especially at the protein level is still lacking. Therefore, to elucidate potato proteome response to PI, we invest ...[more]

PMID: 33297944

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Project description:Systems responses of mature leaves from 4 reference cultivars of a larger collection of European potato cultivars (Solanum tuberosum L.) are investigated by metabolome profiling and RNA-Sequencing. The chosen reference cultivars, Milva, Alegria, Desiree, and Saturna, vary in ascending order in regard to drought tolerance. Systems analyses are based on 3 independent field trials and 3 paralleled greenhouse trials. Robust responses across all cultivars and conditions to natural seasonal drought stress comprise proline, raffinose, galactinol, arabitol, arabinonic acid, chlorogenic acid, and 102 transcripts which consist to a high proportion of heat shock proteins and genes with signaling or regulatory functions, such as a homolog of abscisic acid receptor PYL4. Constitutive differences of the tolerant cultivars, Desiree and Saturna, compared to the sensitive cultivars include arbutin (hydroquinone-beta-D-glucopyranoside), octopamine (p-hydroxyphenylethanolamine), ribitol and 248 differential transcripts. Many of these transcripts are disease related, receptor kinases, or regulatory genes, for example a homolog of the Arabidopsis FOUR LIPS MYB-regulator of stomatal cell proliferation. Functional enrichment analyses imply that heat stress is a major acclimation component of potato leaves to agronomical relevant drought stress. Enhanced leaf heat stress is a result of drought caused by loss of transpiration cooling. This effect and CO2-limitation are the main dilemmas of drought- or ABA-induced stomatal closure. Constitutive differences between tolerant and sensitive cultivars indicate partially synergistic interactions of drought and biotic stress responses. We suggest that drought tolerance of the potato reference cultivars may be caused by general resistance mechanisms which are part of previously selected pathogen tolerance. Transcriptome profiling by RNA-sequencing of 48 leaf samples from 4 potato cultivars grown under control or drought stress conditions in 6 independent experiments

Dataset Information

Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease

Publications

Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease.

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