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Proteome analyses of low phosphorus responses in Stylosanthes guianensis roots

ABSTRACT: Stylosanthes guianensis (stylo) is a pioneer tropical legume with great potential for low phosphorus (P) tolerance. In this study, a P-efficient stylo genotype was used to investigate the protein profiles response to P deficiency through a label-free proteomic approach. A total of 3387 proteins were identified in roots under two P (high or low P) treatments. Proteins with significant changes in abundance (P < 0.05) by more than two-fold were considered as differentially expressed proteins (DEPs). Results showed that 415 proteins displayed differentially expressed under two P treatments, which accounted for 12.3% of the total proteins. These DEPs included 168 up-regulated proteins, which consisted of 117 increased and 51 induced proteins, and 247 down-regulated proteins, including 101 decreased and 146 suppressed proteins. A total of 38 phosphoesterases were identified in stylo roots and could be divided into five categories, such as phosphatase, phosphodiesterase, pyrophosphatase, nuclease and phospholipase. Among them, the phosphatase was the largest group contained 11 DEPs, including eight purple acid phosphatases (PAPs), two protein phosphatase 2A and one inositol monophosphatase. Interestingly, three out of eight PAPs were increased while the other five PAPs were induced in P-deficient stylo roots. These results suggested that PAP proteins play critical roles in stylo adaptation to Pi starvation.

ORGANISM(S): Stylosanthes Guianensis

SUBMITTER: Dr. Pandao Liu

PROVIDER: PXD014704 | iProX | Tue Jul 23 00:00:00 BST 2019

REPOSITORIES: iProX

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Project description:In order to clarify the molecular mechanisms of drought tolerance between different maize (Zea mays L.) varieties at the protein level, iTRAQ (the isobaric tags for relative and absolute quantitation) quantitative proteomics were used for the comparative analysis of protein expression in the seedling roots of the drought-tolerant Chang 7-2 and drought-sensitive TS141 maize varieties under 20% PEG 6000 (polyethylene glycol 6000)-simulated drought stress. A total of 7723 proteins were identified using iTRAQ in the maize seedling roots. We detected 39371 peptides and 30148 unique peptides. The identified protein molecular mass was mainly distributed between 10–60 kDa. COG analysis divided these proteins into 24 categories, among which general function prediction contained the largest number of proteins (19.1%), followed by posttranslational modification, protein turnover, and chaperones (10.52%). In the identified differentially expressed proteins, 1552 of which were significantly differentially expressed. Of these, 1249 were differentially expressed in Chang 7-2 following drought stress, 577 of which were up-regulated and 672 were down-regulated. Four hundred and twenty five differentially expressed proteins were identified in TS141, 249 of which were up-regulated and 176 were down-regulated. Of these proteins, 32 demonstrated opposite expression levels in the two varieties, and there were 56 up-regulated proteins that were common between the two varieties. Comparing the Chang 7-2 treatment group and control group, the DEPS in the cellular component category were mainly enriched in cytoplasmic membrane-bounded vesicles, cytoplasmic vesicles, membrane-bound vesicles and vesicles; in the molecular function category, the DEPs were mainly enriched in cation binding and metal ion binding; and in the biological process category, the major enriched categories included phosphate-containing compound metabolic process, single-organism transport cellular component organization or biogenesis, carbohydrate metabolic process, and cellular component organization. Comparing the TS141 treatment group and control group, the DEPS in the cellular component category were primarily enriched in cytoplasmic membrane-bounded vesicles, cytoplasmic vesicles, membrane-bound vesicles, and vesicle; in the molecular function category, the DEPs were mainly enriched in cation binding and metal ion binding; and in the biological process category, the major enrichment categories included oxidation-reduction process and carbohydrate metabolic process. In Chang 7-2, the differentially expressed proteins were associated with ribosome pathway, glycolysis/gluconeogenesis pathway, and amino sugar and nucleotide sugar metabolism. In TS141, the differentially expressed proteins were associated with metabolic pathway, phenylpropanoid biosynthesis pathway, and starch and sucrose metabolism.

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Proteome analyses of low phosphorus responses in Stylosanthes guianensis roots

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