Dataset Information
CRISPR-Assisted Detection of RNA-Protein Interactions in Living Cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: 
Liang Zhang
PROVIDER: PXD015702 | iProX | Wed May 20 00:00:00 BST 2020
REPOSITORIES: iProX
Publications
CRISPR-assisted detection of RNA-protein interactions in living cells.
Nature methods 20200622 7
We have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA XIST, and it captured a list of known interacting proteins and multiple previously uncharacterized binding proteins. We generalized CARPID to explore binders of the lncRNAs DANCR and MALAT1, revealin ...[more]
