Proteome alterations in brains of KKAy diabetic mice
Ontology highlight
ABSTRACT: Here, we performed a differential proteomic study of brain tissues in HFD KKAy mice andwild type C57BL/6J mice that have the same genetic background. The 18O-labeling technique was employed for protein quantification by mass spectrometry.
Project description:The present study aimed to examine the effect of high-fat diet prior to pregnancy on the liver of mouse offspring. Female C57BL/6J mice were fed a normal chow (15.2% fat by energy) (CTR and CTR-PP groups) or a high-fat chow (31.2% fat by energy) (HFD and HFD-PP groups) for 3−4 weeks and then mated with male C57BL/6J mice fed normal chow. Some mothers continued on the same diet until pups reached 21 days of age (CTR and HFD), and others were fed the different chows from gestational day 0 (CTR-PP and HFD-PP) to determine the effects of a high-fat diet during the pre-pregnancy period in HFD-PP/CTR and HFD/CTR-PP comparisons.
Project description:Mice are on a C57Bl/6J background strain, hearts were collected from Clock and WT mice at ZT07, following 24 weeks on either a HF or SC diet. The microarray approach allows the investigation of gene expression changes of all genes in Clock HFD vs. Clock SC vs. WT HFD vs. WT SC hearts.
Project description:We are exploring pathways influenced by short-term dietary methionine restriction (MR) in thoracic aorta and associated perivascular adipose tissue (PVAT) from obese C57BL/6J male mice around 26 weeks of age. Mice were fed 60% high fat diet (HFD) until they were obese, experimental mice were given 60% HFD with 0.12% methionine for 3, 5, and 10 days while the control group maintained a 60% HFD with 0.86% methionine. All mice were euthanized on the same day, then thoracic aorta was harvested, the PVAT was removed, and both sample types were prepared and analyzed separately. Three mice per group were assessed with LC-MS/MS. MR diet induced lean phenotype in PVAT, which was observed histologically, and we hypothesized that changes in lipid metabolism and mitochondrial bioenergetics mediated this response.
Project description:To understand how diabetes alters the protein subunits of mitochondrial Electron Transfer Chain (ETC) in the podocytes, we performed a proteomic analysis of mitochondrial proteins isolated from primary kidney podocytes of WT (C57BL/6J background) and Ins2Akita/+ diabetic (C57BL/6J background) mice.
Project description:To understand how diabetes alters the protein subunits of mitochondrial Electron Transfer Chain (ETC) in the podocytes, we performed a proteomic analysis of mitochondrial proteins isolated from primary kidney podocytes of WT (C57BL/6J background) and Ins2Akita/+ diabetic (C57BL/6J background) mice.
Project description:The aim of this study was to elucidate the impact of ATP7B deficiency on hepatic gene expression profiles in mice fed a high-fat diet. Systemic ATP7B-knockout (Atp7b-/-) mice on a C57BL/6J genetic background were generated via CRISPR/Cas9 technology and subjected to a high-fat, high-fructose, and high-cholesterol diet (abbreviated as high-fat diet, HFD) for 24 weeks to establish a steatohepatitis model. Hepatic gene-expression profiles were analyzed by RNA sequencing (RNA-seq). ATP7B deficiency exacerbated hepatic inflammation and fibrosis in HFD-fed mice, as revealed by RNA-seq data.
Project description:Effects of EPA/DHA enriched high fat diets (HFD) compared to HFD-Corn oil after 8 weeks intervention on different adipose depots of C57BL/6J mice.
Project description:The present study aimed to examine the effect of high-fat diet prior to pregnancy on the liver of mouse offspring. Female C57BL/6J mice were fed a normal chow (15.2% fat by energy) (CTR and CTR-PP groups) or a high-fat chow (31.2% fat by energy) (HFD and HFD-PP groups) for 3−4 weeks and then mated with male C57BL/6J mice fed normal chow. Some mothers continued on the same diet until pups reached 21 days of age (CTR and HFD), and others were fed the different chows from gestational day 0 (CTR-PP and HFD-PP) to determine the effects of a high-fat diet during the pre-pregnancy period in HFD-PP/CTR and HFD/CTR-PP comparisons. RNA sample was taken from liver of 3-week-old mouse prenatally received high-fat diet prior to pregnancy, during pregnancy and lactation, or through prior to and during pregnancy and lactation, while control RNA was taken from control counterpart prenatally received normal diet alone. Comparisons among groups were made by one-color method with normalized data from Cy3 channels for data analysis.