Membrane protein landscape of plant nuclear envelope and nuclear pore complex

ABSTRACT: We used biochemical fractionation coupled with the subtractive proteomics to profile PNET proteins. Both the crude NE and the total MM fraction were subject to label-free quantitative mass spectrometry (LFQMS). We also adapted proximity labeling-coupled LFQMS (PL-LFQMS) method to map the PNET proteome. Overall, We identified over 200 potential candidates for plant nuclear envelope transmembrane (PNET) proteins and confirmed 14 PNETs.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Yangnan Gu  

PROVIDER: PXD015919 | iProX | Wed Mar 11 00:00:00 GMT 2020

REPOSITORIES: iProX