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Membrane protein landscape of plant nuclear envelope and nuclear pore complex

ABSTRACT: We used biochemical fractionation coupled with the subtractive proteomics to profile PNET proteins. Both the crude NE and the total MM fraction were subject to label-free quantitative mass spectrometry (LFQMS). We also adapted proximity labeling-coupled LFQMS (PL-LFQMS) method to map the PNET proteome. Overall, We identified over 200 potential candidates for plant nuclear envelope transmembrane (PNET) proteins and confirmed 14 PNETs.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Yangnan Gu

PROVIDER: PXD015919 | iProX | Wed Mar 11 00:00:00 GMT 2020

REPOSITORIES: iProX

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Publications

Global profiling of plant nuclear membrane proteome in Arabidopsis.

Tang Yu Y Huang Aobo A Gu Yangnan Y

Nature plants 20200629 7

The nuclear envelope (NE) is structurally and functionally vital for eukaryotic cells, yet its protein constituents and their functions are poorly understood in plants. Here, we combined subtractive proteomics and proximity-labelling technology coupled with quantitative mass spectrometry to understand the landscape of NE membrane proteins in Arabidopsis. We identified ~200 potential candidates for plant NE transmembrane (PNET) proteins, which unravelled the compositional diversity and uniqueness ...[more]

PMID: 32601417

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