The Dynll1-Cox4il Complex Regulates Intracellular Pathogen Clearance via Release of Mitochondrial Reactive Oxygen Species

ABSTRACT: Cellular membrane proteins are a critical part of the host defense mechanisms against infection and intracellular survival of Listeria monocytogenes. The complex spatiotemporal regulation of bacterial infection by various membrane proteins has been challenging to study. Here, using mass spectrometry analyses, we depicted the dynamic expression landscape of membrane proteins upon L. monocytogenes infection in dendritic cells. We showed that Dynein light chain 1 (Dynll1) formed a persistent complex with the mitochondrial cytochrome oxidase, Cox4i1 that is disturbed by pathogen insult. We discovered that the dissociation of the Dynll1-Cox4i1 complex is required for the release of mitochondrial reactive oxygen species and serves as a regulator of intracellular proliferation of Listeria monocytogenes. Our study shows that Dynll1 is an inhibitor of mitochondrial reactive oxygen species and can serve as a potential molecular drug target for antibacterial treatment.

ORGANISM(S): Mus Musculus

SUBMITTER: Ying Wan  

PROVIDER: PXD016452 | iProX | Mon Feb 17 00:00:00 GMT 2020

REPOSITORIES: iProX