Project description:Transcriptional profiling of COLO 320 xenograft tumor cells comparing control COLO 320 xenograft without co-implanted rat MSCs with COLO 320 xenograft with co-implanted rat MSCs. The latter makes co-implanted MSCs visualization possible by using MSCs labeled by GFP under FACS and single cell microscopy. Two-condition experiment, COLO 320 xenograft without rat MSCs [COLO320 MSC(-)] vs. COLO 320 xenograft with rat MSCs [COLO320 MSC(+)]. Biological replicates: 1 control, 1 sample, paired xengraft tumor cells grown and harvested from the same mouse host. One replicate per array.
Project description:Transcriptional profiling of COLO 320 xenograft tumor cells comparing control COLO 320 xenograft without co-implanted rat MSCs with COLO 320 xenograft with co-implanted rat MSCs. The latter makes co-implanted MSCs visualization possible by using MSCs labeled by GFP under FACS and single cell microscopy.
Project description:RNA-seq for MSCs from human bone marrow (BM-MSCs), olfactory mucosa (OM-MSCs), and umbilical cord (UC-MSCs) (2 donors for each). RNA-sequencing for human naïve CD3+ T cells, CD3+ T cells activated with PHA (2.5 μg/mL) for 48 h, and CD3+ T cells co-cultured with human UC-MSCs in the presence of PHA for 48 h.
Project description:The instrinsic regenerative capacity of human fetal cardiac mesenchymal stromal cells (MSCs) has not been fully characterised. Here we demonstrate that we can expand cells with characteristics of cardiovascular progenitor cells from the MSC population of human fetal hearts with only minor fluctuations over time in culture (from day 15 to day 48). We used microarray to compare the gene-expression profile of cultured human fetal cardiac MSCs over time (from day 15 to day 48). MSCs from human fetal hearts were cultured on GelTrex in a defined medium stimulating the canonical Wnt/beta-catenin pathway. Samples from three different time points (day 15, 27 and 48) were compared on microarray.
Project description:The instrinsic regenerative capacity of human fetal cardiac mesenchymal stromal cells (MSCs) has not been fully characterised. Here we demonstrate that we can expand cells with characteristics of cardiovascular progenitor cells from the MSC population of human fetal hearts with only minor fluctuations over time in culture (from day 15 to day 48). We used microarray to compare the gene-expression profile of cultured human fetal cardiac MSCs over time (from day 15 to day 48).
Project description:Transcriptional profiling of human MSCs comparing control MSCs with parathyroid hormone (PTH)-stimulated MSCs. PTH-stimulated MSCs were treated with 0.1 nM recombinant human PTH (N-terminal fragment, amino acids 1-34) for 48 hours. Human MSCs were isolated from a bone marrow sample obtained from a healthy adult volunteer.
Project description:Transcriptional profiling of human MSCs comparing control MSCs with parathyroid hormone (PTH)-stimulated MSCs. PTH-stimulated MSCs were treated with 0.1 nM recombinant human PTH (N-terminal fragment, amino acids 1-34) for 48 hours. Human MSCs were isolated from a bone marrow sample obtained from a healthy adult volunteer. Two-condition experiment: control MSCs vs. PTH-stimulated MSCs. 1 control MSCs and 1 PTH-stimulated MSCs.
Project description:Human primary mesenchymal stromal cells (MSCs) were either left untreated, treated with 3 µg/ml/day GRN, or co-cultured with CD19-sorted CLL cells of two different patients for 5 days. Gene expression of the MSCs was quantified using an Illumina HumanHT-12 v4 Expression BeadChip. Thereby, genes assigned to 454 Illumina probes were identified to be significantly altered by CLL co-culture compared to control MSCs cultured alone (analysed dataset provided). MSCs in co-culture with CLL cells resembled a CAF-like phenotype. There were no significant transcriptional changes detected in GRN treated compared to untreated MSCs.
Project description:A total of 1× 107 cultured UCA-PSCs and WJ-MSCs at P4 were lysed with 300 μL of SDS lysis buffer containing protease inhibitor and 1 μM PMSF in 1.5-mL tubes. Each sample was ultrasonicated on ice for 3 min. After centrifugation twice at 2000 ×g for 10 min at 4°C, the supernatant was collected for total protein measurement and further LC-MS analysis. Cell supernatants (5 mL/106 cells) of P4 UCA-PSCs and WJ-MSCs cultured in basic DMEM without serum or antibiotics for 48 h were collected. After concentration in 3-KD ultrafiltration centrifuge tubes, the cell supernatants were frozen and resuspended in SDS solution. After centrifugation, the supernatants from UCA-PSC and WJ-MSC cultures were obtained.Secretory proteome of UCA-PSCs and WJ-MSCs was further analyzed.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.