Proteomics

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Proteome of the fungus Phoma macdonaldii, the causal agent of black stem in sunflower


ABSTRACT: Phoma macdonaldii causes black stem in sunflower, which severely affects both the yield and quality of sunflowers including weight and oil content of seeds. In this study, a global proteomic analysis of P. macdonaldii was performed in order to facilitate a better understand of the biological and pathogenic characteristics of this fungus. A total of 1,498 proteins were identified by LC-MS/MS in all biological replicates. The identified proteins were classified into functional categories, which included biological process, cellular components and molecular function (GO analysis), metabolism, genetic information progressing, environmental information progressing, cellular processes and organismal systems (KEGG). In addition, the expression of eight genes associated with fungal development and pathogenicity was further investigated. To the best of our knowledge, this is the first study to characterize the proteome of P. macdonaldii. Results from this study provide novel insights into fungal development and pathogenesis of P. macdonaldii and, perhaps even other Phoma species and may contribute to an enhanced understanding the sunflower and P. macdonaldii pathosystem

ORGANISM(S): Plenodomus Lindquistii

SUBMITTER: Yue Liang  

PROVIDER: PXD017157 | iProX | Fri Jan 17 00:00:00 GMT 2020

REPOSITORIES: iProX

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Proteome of the fungus Phoma macdonaldii, the causal agent of black stem of sunflower.

Sun Huiying H   Kav Nat N V NNV   Liang Yue Y   Sun Lin L   Chen Weimin W  

Journal of proteomics 20200612


Phoma macdonaldii causes black stem of sunflower, which severely affects sunflower yield and quality. There is currently little molecular information available for this pathogenic fungus. In this study, a global proteomic analysis of P. macdonaldii was performed to determine the biological characteristics and pathogenicity of this pathogen. A total of 1498 proteins were identified by LC-MS/MS in all biological replicates. Among the identified proteins, 1420 proteins were classified into the thre  ...[more]

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