Dataset Information

Proteome of root from Gossypium barbadense infected by Verticillium

ABSTRACT: In this study, two kinds of island cotton materials with different resistances of Verticillium dahliae were used for Verticillium dahliae infection, the proteomic was used to detect all the protein expression levels in the roots of the two kinds of cotton, and the spectronaut pulsar x software was selected to analyze the data

ORGANISM(S): Gossypium Barbadense

SUBMITTER: Xiang Jin

PROVIDER: PXD017527 | iProX | Sun May 31 00:00:00 BST 2020

REPOSITORIES: iProX

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Publications

Comparative Proteomic Analysis Reveals the Ascorbate Peroxidase-Mediated Plant Resistance to Verticillium dahliae in Gossypium barbadense.

Lu Tianxin T Zhu Liping L Liang Yuxuan Y Wang Fei F Cao Aiping A Xie Shuangquan S Chen Xifeng X Shen Haitao H Wang Beini B Hu Man M Li Rong R Jin Xiang X Li Hongbin H

Frontiers in plant science 20220519

In previous research on the resistance of cotton to Verticillium wilt (VW), Gossypium hirsutum and G. barbadense were usually used as the susceptible and resistant cotton species, despite their different genetic backgrounds. Herein, we present data independent acquisition (DIA)-based comparative proteomic analysis of two G. barbadense cultivars differing in VW tolerance, susceptible XH7 and resistant XH21. A total of 4,118 proteins were identified, and 885 of them were diffe ...[more]

PMID: 35665163

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Project description:Global gene expression was compared between roots of cotton plants (variety Sicot 71) flooded for 4 hours and roots of unflooded cotton plants. Global gene expression was also compared between leaves of cotton plants (variety Sicot 71) flooded for 24 hours and leaves of unflooded cotton plants. Waterlogging stress causes yield reductions in cotton (Gossypium hirsutum L.). A major component of waterlogging stress is the lack of oxygen available to submerged tissues. While changes in expressed protein, gene transcription and metabolite levels have been studied in response to low oxygen stress, little research has been done on molecular responses to waterlogging in cotton. We assessed cotton growth responses to waterlogging and assayed global gene transcription responses in root and leaf cotton tissues of partially submerged plants. Waterlogging causes significant reductions in stem elongation, shoot mass, root mass, and leaf number. At the global gene expression level waterlogging significantly alters the expression of 1012 genes (4.2% of genes assayed) in root tissue as early as 4h after flooding. Many of these genes are associated with cell wall modification and growth pathways, glycolysis, fermentation, mitochondrial electron transport and nitrogen metabolism. Waterlogging of plant roots also altered global leaf gene expression, significantly changing the expression of 1305 genes (5.4% of genes assayed) after 24h of flooding. Genes associated with cell wall growth and modification, tetrapyrrole synthesis, hormone response, starch metabolism and nitrogen metabolism were affected in leaf tissues of waterlogged plants. Implications of these results for the development of waterlogging tolerant cotton are discussed. Keywords: Stress Response Plants of cotton cultivar Sicot 71 were grown to the two-leaf stage in tubs. For stress treatments plants were either watered as normal or flooded with water to completely submerge the root system. At four and twenty-four hours post-flooding samples of root or leaf tissue were taken from control and flooded plants. Total RNA was extracted from each tissue sample and assayed on cotton Affymetrix chips. Two biological replications were used for each comparison.

Dataset Information

Proteome of root from Gossypium barbadense infected by Verticillium

Publications

Comparative Proteomic Analysis Reveals the Ascorbate Peroxidase-Mediated Plant Resistance to <i>Verticillium dahliae</i> in <i>Gossypium barbadense</i>.

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