HDL Quality Features Revealed by Proteome-Lipidome Connectivity are Associated with Atherosclerotic Disease

ABSTRACT: The “quality” rather than “quantity” of lipoprotein particles is more relevant to cardiovascular diseases (CVD), since lipoprotein particles are highly heterogeneous. However, the targeted separation, detection, and exploration of the heterogeneous on omic-scale for lipoprotein remain challenging and rarely touched. Here, we established a high-resolution proteomics and lipidomics (HiPL) method. Briefly, the lipoprotein particles were separated into nine fractions (Lipo-HiPL) using anion-exchange chromatography (AEC), in which HDL, LDL, and VLDL were fractionated into three more fractions respectively. Furthermore, the HDL subclasses were delicately purified within nine fractions (HDL-HiPL), covering diverse protein-lipid heterogeneity. Integration of proteomic and lipidomic analysis revealed proteome-lipidome connectivity (PLC) in lipoprotein and HDL particles, which highly correlated with the atherosclerotic phenotype in rabbit model. Importantly, the lipoprotein “quality” revealed by PLC was found to be related more closely to clinical status in acute coronary syndrome patients than direct proteome and lipidome quantification. Thus, proteome-lipidome connectivity (PLC) as novel HDL “quality” features could be highly associated with atherosclerotic cardiovascular diseases.

ORGANISM(S): Homo Sapiens

SUBMITTER: Rong Zeng  

PROVIDER: PXD017529 | iProX | Sat Feb 15 00:00:00 GMT 2020

REPOSITORIES: iProX