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Proteins differentially expressed during limonene biotransformation by Penicillium digitatum DSM 62840 were examined using iTRAQ labeling coupled with 2D‑LC–MS/MS

ABSTRACT: In this study, a relative quantitative comparison of proteins that are differentially expressed at two time points during limonene biotransformation (0 and 12 h) is performed using iTRAQ combined with LC–MS/MS. It provides a basis for further study of the molecular mechanism of limonene biotransformation using Penicillium digitatum DSM 62840.

ORGANISM(S): Penicillium Digitatum

SUBMITTER: Gang Fan

PROVIDER: PXD017752 | iProX | Wed Feb 26 00:00:00 GMT 2020

REPOSITORIES: iProX

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Genomic and Transcriptomic Study for Screening Genes Involved in the Limonene Biotransformation of <i>Penicillium digitatum</i> DSM 62840.

Zhang Lu-Lu LL Huang Wen W Zhang Ying-Ying YY Fan Gang G He Jin J Ren Jing-Nan JN Li Zhi Z Li Xiao X Pan Si-Yi SY

Frontiers in microbiology 20200422

α-Terpineol has been widely used in daily chemical, pharmaceutical, food, and flavor industries due to its pleasant odor with high economic value and pharmacological action. Our previous study showed that <i>Penicillium digitatum</i> DSM 62840 was an efficient biocatalyst for the transformation of limonene to α-terpineol. Thus, it was meaningful to explore the genome features and the gene expression differences of strain DSM 62840 during limonene biotransformation, and the detailed bioconversion ...[more]

PMID: 32390984

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