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Genome-wide targets identification for high-titer production of free fatty acids in Escherichia coli

ABSTRACT: We employed omics analyses to identify potential targets in multiple cellular processes, enabling systematical discovery of beneficial chromosomal gene targets that can be engineered to optimize free fatty acids (FFAs) production in Escherichia coli.

ORGANISM(S): Escherichia Coli

SUBMITTER: Hao Song

PROVIDER: PXD017890 | iProX | Wed Jun 03 00:00:00 BST 2020

REPOSITORIES: iProX

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Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids.

Fang Lixia L Fan Jie J Luo Shulei S Chen Yaru Y Wang Congya C Cao Yingxiu Y Song Hao H

Nature communications 20210817 1

To construct a superior microbial cell factory for chemical synthesis, a major challenge is to fully exploit cellular potential by identifying and engineering beneficial gene targets in sophisticated metabolic networks. Here, we take advantage of CRISPR interference (CRISPRi) and omics analyses to systematically identify beneficial genes that can be engineered to promote free fatty acids (FFAs) production in Escherichia coli. CRISPRi-mediated genetic perturbation enables the identification of 30 ...[more]

PMID: 34404790

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