Dataset Information

ITRAQ protein profile of sugar beet (Beta vulgaris)

ABSTRACT: Leaves and roots were used to identify the differentially abundant protein species between salt-stress and control conditions in beta vulgaris. The experimental material was a salt-tolerant beet strain 'o68'.Salt treatment was carried out by gradual salt feeding. Samples were collected at 24h after the concentration reached 300mM.

ORGANISM(S): Beta Vulgaris

SUBMITTER: Jie Cui

PROVIDER: PXD017954 | iProX | Mon Mar 09 00:00:00 GMT 2020

REPOSITORIES: iProX

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Publications

iTRAQ protein profile analysis of sugar beet under salt stress: different coping mechanisms in leaves and roots.

Li Junliang J Cui Jie J Cheng Dayou D Dai Cuihong C Liu Tianjiao T Wang Congyu C Luo Chengfei C

BMC plant biology 20200722 1

<h4>Background</h4>Salinity is one of the most serious threats to world agriculture. An important sugar-yielding crop sugar beet, which shows some tolerance to salt via a mechanism that is poorly understood. Proteomics data can provide important clues that can contribute to finally understand this mechanism.<h4>Results</h4>Differentially abundant proteins (DAPs) in sugar beet under salt stress treatment were identified in leaves (70 DAPs) and roots (76 DAPs). Functions of these DAPs were predict ...[more]

PMID: 32698773

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Project description:Background: Soil salinity is a major abiotic stress factor that limit agricultural productivity worldwide, and this problem is expected to grow in the future. Common bean (Phaseolus vulgaris L.) is an important protein source in developing countries is highly susceptible to salt stress. To understand the underlying mechanism of salt stress responses, transcriptomics, metabolomics, and ion content analysis were utilized for response comparison of salt-tolerant and salt-susceptible common bean genotypes in saline conditions. Results: Transcriptome analysis has revealed that the tolerant genotype had increased photosynthesis in saline conditions while the susceptible genotype acted in a contrasting way. The chlorophyll content measurements have backed up this result with increase in tolerant and decrease in susceptible genotype. Transcriptome also displayed a more active carbon and amino acid metabolism for the tolerant genotype as well. Analysis of primary metabolites with GC-MS demonstrated the boosted carbohydrate metabolism in the tolerant genotype with increased sugar content as well as better amino-acid metabolism with the accumulation of glutamate and asparagine and hinted a lowered photorespiration level for the tolerant one. Accumulation of lysine, valine, and isoleucine in the roots of the susceptible genotype suggested a halted stress response pathway. According to ion content comparison, the tolerant genotype managed to block accumulation of Na+ in the leaves while accumulating significantly less Na+ in the roots compared to susceptible genotype. K+ levels increased in the leaves of both genotype and the roots of the susceptible one but dropped in the roots of the tolerant genotype. Additionally, Zn+2 and Mn+2 levels were also dropped in the tolerant roots, while Mo+2 levels were significantly higher in all tissues in both control and saline conditions for tolerant genotype. Conclusion:The results of the presented study have demonstrated the differences in contrasting genotypes and thus provide valuable information on the pivotal molecular mechanisms underlying salt tolerance mainly in common bean, but for all crops.

Dataset Information

ITRAQ protein profile of sugar beet (Beta vulgaris)

Publications

iTRAQ protein profile analysis of sugar beet under salt stress: different coping mechanisms in leaves and roots.

Similar Datasets

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