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ITRAQ of Antrodia camphorata S-29

ABSTRACT: Protein quantification by iTRAQ was carried out according to the method of Lu et al. (2017). Equally mixed peptides were analyzed by Majorbio Biopharm Technology Co., Ltd. The mixed peptides were preseparated via a C18 reversed-phase column and analyzed via liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

ORGANISM(S): Taiwanofungus Camphoratus

SUBMITTER: Yongjun Xia

PROVIDER: PXD018004 | iProX | Wed Mar 11 00:00:00 GMT 2020

REPOSITORIES: iProX

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Comprehensive transcriptomic and proteomic analyses of antroquinonol biosynthetic genes and enzymes in Antrodia camphorata.

Liu Xiaofeng X Xia Yongjun Y Zhang Yao Y Yang Caiyun C Xiong Zhiqiang Z Song Xin X Ai Lianzhong L

AMB Express 20200803 1

Antroquinonol (AQ) has several remarkable bioactivities in acute myeloid leukaemia and pancreatic cancer, but difficulties in the mass production of AQ hamper its applications. Currently, molecular biotechnology methods, such as gene overexpression, have been widely used to increase the production of metabolites. However, AQ biosynthetic genes and enzymes are poorly understood. In this study, an integrated study coupling RNA-Seq and isobaric tags for relative and absolute quantitation (iTRAQ) we ...[more]

PMID: 32748086

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