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Escherichia coli-based cell-free reactions in different oxygen concentrations

ABSTRACT: Escherichia coli-based cell-free protein synthesis reactions conducted in precisely contolled oxygen concentrations within tube-in-tube reactor. The cell-free reactions after 4-hour incubation in 0%, 21%, and 100% oxygen were analysized by HPLC-MS/MS.

ORGANISM(S): Escherichia Coli

SUBMITTER: Yuan Lu

PROVIDER: PXD018058 | iProX | Mon Mar 16 00:00:00 GMT 2020

REPOSITORIES: iProX

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O<sub>2</sub>-Tuned Protein Synthesis Machinery in <i>Escherichia coli</i>-Based Cell-Free System.

Lin Xiaomei X Zhou Caijin C Zhu Songbiao S Deng Haiteng H Zhang Jisong J Lu Yuan Y

Frontiers in bioengineering and biotechnology 20200409

Involved in most aerobic biochemical processes, oxygen affects cellular functions, and organism behaviors. Protein synthesis, as the underlying biological process, is unavoidably affected by the regulation of oxygen delivery and utilization. Bypassing the cell wall, cell-free protein synthesis (CFPS) systems are well adopted for the precise oxygen regulation analysis of bioprocesses. Here a reliable flow platform was developed for measuring and analyzing the oxygen regulation on the protein synt ...[more]

PMID: 32328487

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