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Proteomic Quantification of Lysine Succinylation in Human Gastric Cancer

ABSTRACT: Using TMT labeling and affinity enrichment followed by high-resolution LC-MS/MS analysis, quantitative lysine succintylome analysis was performed.

ORGANISM(S): Homo Sapiens

SUBMITTER: Qiang You

PROVIDER: PXD018376 | iProX | Mon Apr 06 00:00:00 BST 2020

REPOSITORIES: iProX

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Lysine-222 succinylation reduces lysosomal degradation of lactate dehydrogenase a and is increased in gastric cancer.

Li Xiang X Zhang Chen C Zhao Ting T Su Zhongping Z Li Mengjing M Hu Jiancheng J Wen Jianfei J Shen Jiajia J Wang Chao C Pan Jinshun J Mu Xianmin X Ling Tao T Li Yingchang Y Wen Hao H Zhang Xiaoren X You Qiang Q

Journal of experimental & clinical cancer research : CR 20200828 1

<h4>Background</h4>Lysine succinylation is an emerging posttranslational modification that has garnered increased attention recently, but its role in gastric cancer (GC) remains underexplored.<h4>Methods</h4>Proteomic quantification of lysine succinylation was performed in human GC tissues and adjacent normal tissues by mass spectrometry. The mRNA and protein levels of lactate dehydrogenase A (LDHA) in GC and adjacent normal tissues were analyzed by qRT-PCR and western blot, respectively. The ex ...[more]

PMID: 32859246

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