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Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells

ABSTRACT: m6A is the most abundant modification of mRNA in mammals and plays an important role in human development and disease. METTL14 is a key component of m6A methyltransferase complex. The project showed that its post-translational arginine methylation can regulate the generation of m6A and the endoderm differentiation of mouse embryonic stem cells, indicating its essential function in the normal development of embryos.

ORGANISM(S): Homo Sapiens

SUBMITTER: Shan Xiao

PROVIDER: PXD018458 | iProX | Mon May 17 00:00:00 BST 2021

REPOSITORIES: iProX

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Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells.

Liu Xiaona X Wang Hailong H Zhao Xueya X Luo Qizhi Q Wang Qingwen Q Tan Kaifen K Wang Zihan Z Jiang Jia J Cui Jinru J Du Enhui E Xia Linjian L Du Wenyi W Chen Dahua D Xia Laixin L Xiao Shan S

Nature communications 20210618 1

RNA N6-methyladenosine (m6A), the most abundant internal modification of mRNAs, plays key roles in human development and health. Post-translational methylation of proteins is often critical for the dynamic regulation of enzymatic activity. However, the role of methylation of the core methyltransferase METTL3/METTL14 in m6A regulation remains elusive. We find by mass spectrometry that METTL14 arginine 255 (R255) is methylated (R255me). Global mRNA m6A l ...[more]

PMID: 34145242

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