Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

3 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quorum sensing system-regulated protein affect the spoilage potential of co-culturing Acinetobacter johnsonii and Pseudomonas fluorescens from spoilage bigeye tuna (Thunnus obesus)

ABSTRACT: Quorum sensing system-regulated protein affect the spoilage potential of co-culturing Acinetobacter johnsonii and Pseudomonas fluorescens from spoilage bigeye tuna (Thunnus obesus) by proteomics

ORGANISM(S): Pseudomonas Fluorescens Pf0-1 Acinetobacter Johnsonii

SUBMITTER: Jing Xie

PROVIDER: PXD018646 | iProX | Sat Apr 18 00:00:00 BST 2020

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Quorum Sensing System-Regulated Proteins Affect the Spoilage Potential of Co-cultured Acinetobacter johnsonii and Pseudomonas fluorescens From Spoiled Bigeye Tuna (Thunnus obesus) as Determined by Proteomic Analysis.

Wang Xin-Yun XY Xie Jing J

Frontiers in microbiology 20200514

Food spoilage by certain species of bacteria is reported to be regulated by quorum sensing (QS). Acinetobacter johnsonii and Pseudomonas fluorescens, the major specific spoilage organisms, are found to be limited in their QS and co-culture interactions. The aim of this study was to determine how QS-regulated proteins affect the spoilage potential of co-cultured A. johnsonii and P. fluorescens obtained from spoiled bigeye tuna (Thunnus obesus) using a proteomics ...[more]

PMID: 32477317

Similar Datasets

The Fem system in Pseudomonas aeruginosa functions as an ExsA-regulated interspecies signaling pathway

Project description:Bacterial interspecies interactions shape the function and structural dynamics of microbial communities and affect disease progression of polymicrobial infections. Here, we present data suggesting that the FemA-FemR-FemI (Fem) cell surface signaling system in P. aeruginosa serves as an interspecies signaling pathway between P. aeruginosa and Mycobacterium species. The Fem system is regulated by the type three secretion system (T3SS) regulator ExsA. Fem system significantly influenced virulence factors in P. aeruginosa, including the quorum sensing systems, pyocyanin production, biofilm formation and the type six secretion systems (T6SSs). Our study using a Galleria mellonella infection model indicates femA deletion significantly increased the host survival rate while femI over-expression decreased it, demonstrating that the Fem system’s role in bacterial pathogenicity in vivo. We propose that the Fem system functions as an interspecies signaling pathway enabling P. aeruginosa to alter its behaviours in response to the presence of mycobacteria.

2021-01-14 | GSE164773 | GEO

The ParS/ParR regulon in Pseudomonas aeruginosa PAO1

Project description:The ParS/ParR two component regulatory system plays important roles for multidrug resistance in Pseudomonas aeruginosa. In this study we report RNA-seq analyses of the transcriptomes of P. aeruginosa PAO1 wild type and par mutants growing in a minimal medium containing 2% casamino acids. This has allowed the quantification of PAO1 transcriptome, and further defines the regulon that is dependent on the ParS/ParR system for expression. Our RNA-seq analysis produced the first estimates of absolute transcript abundance for the 5570 coding genes in P. aeruginosa PAO1. Comparative transcriptomics of P. aeruginosa PAO1 and par mutants identified a total of 464 genes regulated by ParS and ParR. Results also showed that mutations in the parS/parR system abolished the expression of the mexEF-oprN operon by down-regulating the regulatory gene mexS. In addition to affecting drug resistance genes, transcripts of quorum sensing genes (rhlIR and pqsABCDE-phnAB), were significantly up-regulated in both parS and parR mutants. Consistent with these results, a significant portion of the ParS/ParR regulated genes belonged to the MexEF-OprN and quorum sensing regulons. Deletion of par genes also lead to overproduction of phenazines and increased swarming motility, consistent with the up-regulation of quorum sensing genes. Our results established a link among ParS/ParR, MexEF-OprN and quorum sensing in Pseudomonas aeruginosa. Based on these results, we propose a model to illustrate the relationship among these regulatory systems in P. aeruginosa. A total of 9 samples were analyzed in AB medium + 2% casamino acids, Pseudomonas aeruginosa PAO1 wild type strain (3 replicates); Pseudomonas aeruginosa parS mutant (3 replicates); Pseudomonas aeruginosa parR mutant (3 replicates).

2016-02-26 | E-GEOD-44681 | biostudies-arrayexpress

Phenazine regulated genes in Pseudomonas chlororaphis 30-84

Project description:Pseudomonas chlororaphis strain 30-84 is an effective biological control agent against take-all disease of wheat. Phenazines, bacterial secondary metabolites produced by 30-84, are essential for 30-84 to inhibit fungal pathogens, form biofilms, and effectively colonize the rhizosphere. However, how the bacteria themselves respond to phenazines remains unknown. In this study, we conducted an RNA-seq analysis by comparing the wild type strain with a phenazine deficient mutant. RNA-seq analysis identified over 200 genes differentially regulated by phenazines. Consistent with previous findings in Pseudomonas aeruginosa PAO1, phenazines positively contribute to the expression of their own biosynthetic genes. Moreover, phenazine regulatory genes including the phzI/phzR quorum sensing system and the rpeB response regulatory were also expressed at high levels in the presence of phenazines. Besides phenazine biosynthesis and regulatory genes, genes involved in secondary metabolism, exopoysaccharide production and iron uptake as well as amino acid transport were identified as the major components under phenazine control, including many novel genes. We have also demonstrated that mutation of the primary siderophore gene pvdA resulted in up-regulation of phenazine genes when grown in iron-limiting media. These findings implicate phenazines as signaling molecules to regulate gene expression and hence alter metabolism in P. chlororaphis strain 30-84. A total of 4 samples were analyzed in AB medium + 2% casamino acids, Pseudomonas chlororaphis wild type strain (2 replicates); Pseudomonas chlororaphis ZN mutant (2 replicates).

2014-11-25 | E-GEOD-45247 | biostudies-arrayexpress

Pseudomonas virulence factor controls expression of virulence genes in Pseudomonas entomophila

Project description:Quorum sensing is a communication strategy that bacteria use to collectively alter gene expression in response to cell density. Pathogens use quorum sensing systems to control activities vital to infection, such as the production of virulence factors and biofilm formation. The Pseudomonas virulence factor (pvf) gene cluster encodes a quorum sensing system (Pvf) that is present in over 500 strains of proteobacteria, including strains that infect a variety of plant and human hosts. We have shown that the Pvf quorum sensing system regulates the production of secreted proteins and small molecules in the insect pathogen Pseudomonas entomophila L48. Here, we identified genes that are likely regulated by Pvf using the model strain P. entomophila L48 which does not contain other known quorum sensing systems. Pvf regulated genes were identified through comparing the transcriptomes of wildtype P. entomophila and a pvf deletion mutant (ΔpvfA-D). We found that deletion of pvfA-D affected the expression of approximately 300 genes involved in virulence, the type VI secretion system, siderophore transport, and branched chain amino acid biosynthesis. Additionally, we identified seven putative biosynthetic gene clusters whose expression are reduced in ΔpvfA-D. Our results indicate that Pvf controls multiple virulence mechanisms in P. entomophila L48. Characterizing genes regulated by Pvf will aid understanding of host-pathogen interactions and development of anti-virulence strategies against P. entomophila and other pvf-containing strains.

2023-03-19 | GSE216056 | GEO

Transcriptomics analysis of Aspergillus fumigatus co-cultivated with Pseudomonas aeruginosa

Project description:We report a study conducted to investigate the variation on gene expression of the pathogenic fungus Aspergillus fumigatus upon co-cultivation with the pathogenic bacterium Pseudomonas aeruginosa. The study was conducted by investigating the gene expression variation at different time points (45, 90 and 180 minutes after co-incubation). As control, we used data obtained by cultivating the fungus either without bacteria, or with heat-inactivated Pseudomonas.

2018-12-09 | GSE122391 | GEO

Phenazine regulated genes in Pseudomonas chlororaphis 30-84

2014-11-25 | GSE45247 | GEO

The ParS/ParR regulon in Pseudomonas aeruginosa PAO1

2016-02-26 | GSE44681 | GEO

Novel targets of the CbrAB/Crc carbon catabolite control system revealed by transcript abundance in Pseudomonas aeruginosa.

Project description:This SuperSeries is composed of the following subset Series: GSE33241: Novel targets of the CbrAB/Crc carbon catabolite control system revealed by transcript abundance in Pseudomonas aeruginosa [BSM] GSE33244: Novel targets of the CbrAB/Crc carbon catabolite control system revealed by transcript abundance in Pseudomonas aeruginosa [LB] Refer to individual Series

2012-03-31 | E-GEOD-33245 | biostudies-arrayexpress

Transcriptome data of co-culture of Brevundimonas diminuta R79 and Pseudomonas balearica R90 in domestication medium

Project description:It is a cost-effective production method to synthesize polyhydroxyalkanoate (PHA) through mixed bacterial consortium fermentation. Pseudomonas balearica R90 and Brevundimonas diminuta R79, which represent the species of abundance increase in the propylene oxide activated sludge, were selected as the model to study the interaction mechanism of PHA synthesis by co-culture between strains. RNA-Seq analysis showed that the expression of acs and phaA genes of R79 and R90 increased in the mixed culture group, which enhanced their ability of acetic acid utilization and poly-β-hydroxybutyrate (PHB) synthesis. So the dry cell weight and the yield of PHB in the mixed culture group was prominently higher than that in the pure culture group. In addition, two-component system genes, quorum- sensing genes, flagellar synthesis related genes and chemotaxis related genes in R90 were enriched at 4 days, so R90 could adapt to the domestication environment more quickly, making the proportion of R90 higher than that of R79 at 4 days. However, the acs gene expression of R79 was higher than that of R90. Therefore, R79 can make better utilize acetate in the domestication solution environment, thus occupying the final dominant position.

2022-10-29 | GSE216514 | GEO

Pseudomonas aeruginosa PA14 transcriptome during co-culture Aspergillus fumigatus

Project description:Pseudomonas aeruginosa monocultures and co-cultures with Aspergillus fumigatus during growth in SCFM2 were harvested in the stationary phase of growth (triplicates) by aspirating the contents of the petri-dish well into a 15 ml falcon tube (passed over a 40 µm cell strainer) and combining this with an equal volume of RNA-later. Cultures stored in RNAlater were pelleted, resuspended in 1 mL RNA Bee, and RNA was extracted as described previously.

2023-03-30 | E-MTAB-12786 | biostudies-arrayexpress