Dataset Information

ITRAQ-based proteomic profile analysis of the hepatopancreas of Caribbean spiny lobsters infected with Panulirus argus virus 1: metabolic and physiological implications

ABSTRACT: The Caribbean spiny lobster Panulirus argus (Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virus Panulirus argus Virus 1 (PaV1). To date, there is no available information regarding the host alterations induced by this pathogen at the molecular level. In the present study, comparative proteomic analyses of the changes in the hepatopancreas between infected and non-infected juvenile lobsters were analyzed by isobaric tags for relative and absolute quantitation (iTRAQ) coupled to synchronous precursor selection (SPS)-based MS3. We identified a total of 636 proteins, being 68 down-regulated and 71 up-regulated proteins. Among down-regulated proteins, we identified several enzymes involved in the metabolism of hormones and lipids, digestive proteases and glycosidases, while proteins associated with the histone core, protein synthesis, immune response and RNA regulation were up-regulated, and several misregulated enzymes involved in the regulation of neuromodulators were also identified. RT-qPCR data used to validate the expression of transcripts encoding for differential proteins were in concordance to proteomic data, as well as the tendency observed for trypsin, chymotrypsin, glycosidase enzyme activities. In a similar way, we observed a glycogen reduction in muscle, and an increase in plasma acylglycerides and glucose, which may be explained by proteomic data. This study provides the first insight into the molecular changes in the hepatopancreas of Caribbean spiny lobsters associated to PaV1 infection and the deregulation of proteins implicated in metabolic processes characteristics of the disease in lobsters.

ORGANISM(S): Panulirus Argus

SUBMITTER: Rossanna Rodríguez

PROVIDER: PXD018882 | iProX | Wed Apr 29 00:00:00 BST 2020

REPOSITORIES: iProX

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Publications

iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected With Panulirus argus Virus 1: Metabolic and Physiological Implications.

Zamora-Briseño Jesús Alejandro JA Ruiz-May Eliel E Elizalde-Contreras José Miguel JM Hernández-Velázquez Ioreni Margarita IM Hernández-Pérez Ariadne A Fuentes-García Ana Guadalupe AG Herrera-Salvatierra Nancy N Briones-Fourzán Patricia P Pascual-Jiménez Cristina C Lozano-Álvarez Enrique E Rodríguez-Canul Rossanna R

Frontiers in microbiology 20200529

The Caribbean spiny lobster Panulirus argus (Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virus Panulirus argus Virus 1 (PaV1) that causes a systemic and chronic-degenerative infection in juvenile spiny lobsters P. argus. To date, there is no available information regarding the host alterations induced by this pathogen at the molecular level. In the present study, compara ...[more]

PMID: 32547519

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Project description:Many virus diseases of economic importance to agriculture result from mixtures of different pathogens invading the host at a given time. This contrasts with the relatively scarce studies available on the molecular events associated with virus-host interactions in mixed infections. In comparison to single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) resulted in increased systemic symptoms (synergism) that led to necrosis of the newly emerging leaves, and the plant death. A comparative transcriptional analysis was undertaken to identify quantitative and qualitative differences in gene expression during this synergistic infection, and to correlate these changes with the severe symptoms it caused. Global transcription profiles of doubly-infected leaves were compared with those from singly-infected leaves using gene ontology enrichment analysis and metabolic pathway annotator software. Functional gene categories altered by the double infection comprise suites of genes regulated coordinately, which are associated with chloroplast functions (down-regulated), protein synthesis and degradation (up-regulated), carbohydrate metabolism (up-regulated), and response to biotic stimulus and stress (up-regulated). The expression of reactive oxygen species-generating enzymes as well as several mitogen-activated protein kinases, were also significantly induced. Accordingly, synergistic infection induced a severe oxidative stress in N. benthamiana leaves, as judged by increases in lipid peroxidation, and by the generation of superoxide radicals in chloroplasts, which correlated with the misregulation of antioxidative genes in microarray data. Interestingly, expression of genes encoding oxylipin biosynthesis was uniquely up-regulated by the synergistic infection. Virus-induced gene silencing of alfa-dioxygenase1 delayed cell death during PVX-PVY infection. Using mock inoculated leaf tissue as a reference, we compare the gene expression profiles of Nicotiana benthamiana plants infected with one of two viruses, Potato virus X (PVX) or Potato virus Y (PVY), or the combination PVX plus PVY. 3 biological replicates per treatment were independently grown and haversted.

Dataset Information

ITRAQ-based proteomic profile analysis of the hepatopancreas of Caribbean spiny lobsters infected with Panulirus argus virus 1: metabolic and physiological implications

Publications

iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected With <i>Panulirus argus</i> Virus 1: Metabolic and Physiological Implications.

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