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Multi-in-One: Multiple-proteases, One-hour-shot Strategy for Fast and High-coverage Phosphoproteome

ABSTRACT: a ‘multi-in-one’ strategy to saturate the phosphosite coverage from multiple-proteases by one-step enrichment and one-shot analysis in just over one hour, through data-independent acquisition (DIA)

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Rong Zeng

PROVIDER: PXD019320 | iProX | Sun Jun 07 00:00:00 BST 2020

REPOSITORIES: iProX

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Multi-in-One: Multiple-Proteases, One-Hour-Shot Strategy for Fast and High-Coverage Phosphoproteomic Investigation.

Gao Xiaojing X Li Qingrun Q Liu Yansheng Y Zeng Rong R

Analytical chemistry 20200617 13

Although a multiple-protease based shotgun proteomics method was shown to improve coverage for phosphosite identification, this traditional pipeline is time-consuming and can be of low reproducibility. Here, we demonstrated a multi-in-one strategy to saturate the phosphosite coverage by combining the multiple-proteases based digestion, one-step enrichment, and one-shot data-independent acquisition (DIA) as short as 1 h. In the "three-in-one" workflow, more than 19,700 and 13,500 phosphosites cou ...[more]

PMID: 32479063

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