Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens Escherichia Coli Mus Musculus
SUBMITTER: Tiannan Guo
PROVIDER: PXD019379 | iProX | Sun May 24 00:00:00 BST 2020
REPOSITORIES: iProX
Cai Xue X Ge Weigang W Yi Xiao X Sun Rui R Zhu Jiang J Lu Cong C Sun Ping P Zhu Tiansheng T Ruan Guan G Yuan Chunhui C Liang Shuang S Lyu Mengge M Huang Shiang S Zhu Yi Y Guo Tiannan T
Journal of proteome research 20201008 1
The performance of data-independent acquisition (DIA) mass spectrometry (MS) depends on the separation efficiency of peptide precursors. In Orbitrap-based mass spectrometers, separation efficiency of peptide precursors is limited by the relatively slow scanning rate compared to time of flight (TOF)-based MS. Here, we present PulseDIA, a multi-injection gas-phase fractionation (GPF) strategy for enhanced DIA-MS. This is achieved by equally dividing the conventional DIA analysis covering the entir ...[more]