Dataset Information

Diaphorina citri salivary glands shotgun

ABSTRACT: In order to identify proteins potentially act as salivary effectors, we conducted proteomic analyses in the SG of D. citri using LC−MS/MS. Two hundred pairs of SG from CLas uninfected D. citri clone were dissected out in SDT lysis buffer. LC−MS/MS analysis was conducted on a Q Exactive mass spectrometer (Thermo Fisher Scientific, MA, USA) coupled to Easy nLC (Thermo Fisher Scientific). Our results provide useful resources to identify potential functional proteins like salivary effectors and basic framework for future investigation to understand the molecular interaction between phloem sap-sucking insects and host plants.

ORGANISM(S): Diaphorina Citri

SUBMITTER: Hongbo Jiang

PROVIDER: PXD019624 | iProX | Sat Jun 06 00:00:00 BST 2020

REPOSITORIES: iProX

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Publications

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus.

Liu Xiao-Qiang XQ Jiang Hong-Bo HB Liu Tian-Yuan TY Yang Li L Fan Jia-Yao JY Xiong Ying Y Jing Tian-Xing TX Lou Bing-Hai BH Dou Wei W Wang Jin-Jun JJ

Frontiers in physiology 20200925

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the principal vector of the Candidatus Liberibacter asiaticus (CLas) bacterium that causes Huanglongbing (HLB) disease. The D. citri salivary glands (SG) is an important barrier to the transmission of CLas. Despite its importance, the transcriptome and proteome of SG defense against CLas are unstudied in D. citri. In the present study, we generated a comparative transcriptome dataset of the SG in infected a ...[more]

PMID: 33101062

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Project description:Although the salivary transcriptome of adult mosquitoes has been thoroughly described in several recent papers, very little information exists regarding the biological role of larval salivary glands in the Culicidae. We used whole-transcriptome AffymetrixÂ® chips to compare the transcriptional profiles of Anopheles gambiae larval (L4) salivary glands and whole larvae. We identified a total of 277 transcripts as being significantly enriched in the salivary glands. Based on available annotation for the known or predicted protein sequences encoded by these transcripts, 41 were identified as corresponding to secreted proteins, 233 as corresponding to non-secreted (housekeeping) proteins, and 3 as encoding proteins of unknown function. Based on functional annotation of the putatively secreted gene products, we propose that larval salivary secretions have roles in nutrient digestion, detoxification, immunity, and mouthpart lubrication. Interestingly, several components of the larval saliva (e.g. apyrase and serine proteases) have also been reported to exist in adult female saliva, where they are though to help regulate a vertebrate hostâs immune response to bloodfeeding. In conclusion, our results suggest that the salivary glands are important components of both the digestive and immune systems of larval mosquitoes, and that their study might provide clues about the evolution of adaptations to bloodfeeding observed in adults. Experiment Overall Design: We attempted to identify transcripts that are both present and significantly enriched in the salivary glands (SG) of the 4th instar Anopheles gambiae larva. Transcripts were considered as present in the SG if the built-in Affymetrix detection call scored them as such in all three biological replicates of the experiment. Expression values (i.e. signal intensities) of transcripts from the salivary glands were compared to those of a matching group of whole 4th instar larvae using a t-test. Transcripts showing an expression value two-fold or higher (p < 0.01; false discovery rate=1%) in the SG as compared to whole larvae were considered to be significantly enriched.

Dataset Information

Diaphorina citri salivary glands shotgun

Publications

A Transcriptomic and Proteomic Analysis of the <i>Diaphorina citri</i> Salivary Glands Reveals Genes Responding to <i>Candidatus</i> Liberibacter asiaticus.

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