Secreted proteins of Yersinia pestis 201

ABSTRACT: Y. pestis strain 201 cultures grown under Mh (37°C, TMH with no calcium) and Fv (26°C, TMH with 2.5M calcium) conditions were used as the experimental and control groups, respectively. After 8 h of incubation, the cultures supernatants were collected by centfugation and concentrated using centrifugal filters. The concentrations of proteins were determined using a Pierce BCA Protein Assay Kit. Tandem mass tag labeling was used to obtain accurate quantitative information to minimize variability between samples. For quantitative analysis, at least two unique peptides were used for the identification and quantification of proteins.

ORGANISM(S): Yersinia Pestis Co92

SUBMITTER: Ruifu Yang  

PROVIDER: PXD020062 | iProX | Wed Dec 16 00:00:00 GMT 2020

REPOSITORIES: iProX