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Identification of the acetylation sites in ALKBH5

ABSTRACT: The acetylation modification sites in ALKBH5 was identified by mass spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Guangrong Yan

PROVIDER: PXD020070 | iProX | Thu Apr 13 00:00:00 BST 2023

REPOSITORIES: iProX

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K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis.

Zhang Xiao-Lan XL Chen Xin-Hui XH Xu Binwu B Chen Min M Zhu Song S Meng Nan N Wang Ji-Zhong JZ Zhu Huifang H Chen De Liu Jin-Bao JB Yan Guang-Rong GR

Nature communications 20230627 1

N6-methyladenosine (m6A) modification plays important roles in bioprocesses and diseases. AlkB homolog 5 (ALKBH5) is one of two m6A demethylases. Here, we reveal that ALKBH5 is acetylated at lysine 235 (K235) by lysine acetyltransferase 8 and deacetylated by histone deacetylase 7. K235 acetylation strengthens the m6A demethylation activity of ALKBH5 by increasing its recognition of m6A on mRNA. RNA-binding protein paraspeckle component 1 (PSCP1) is a r ...[more]

PMID: 37369679

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