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PCT2G_15min_HCC_25patients_115_files

ABSTRACT: PCT2G_15min_HCC_SCIEX6600_115_files_gaohuanhuan_20191112

ORGANISM(S): Homo Sapiens

SUBMITTER: TiannanGuo

PROVIDER: PXD020348 | iProX | Tue Jul 14 00:00:00 BST 2020

REPOSITORIES: iProX

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Publications

Accelerated Lysis and Proteolytic Digestion of Biopsy-Level Fresh-Frozen and FFPE Tissue Samples Using Pressure Cycling Technology.

Gao Huanhuan H Zhang Fangfei F Liang Shuang S Zhang Qiushi Q Lyu Mengge M Qian Liujia L Liu Wei W Ge Weigang W Chen Chen C Yi Xiao X Zhu Jiang J Lu Cong C Sun Ping P Liu Kexin K Zhu Yi Y Guo Tiannan T

Journal of proteome research 20200330 5

Pressure cycling technology (PCT)-assisted tissue lysis and digestion have facilitated reproducible and high-throughput proteomic studies of both fresh-frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue of biopsy scale for biomarker discovery. Here, we present an improved PCT method accelerating the conventional procedures by about two-fold without sacrificing peptide yield, digestion efficiency, peptide, and protein identification. The time required for processing 16 tissue samples ...[more]

PMID: 32182071

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